Detailed information of TA system TA122312

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 26469..26722
• Replicon: plasmid pDEC5B-4
• Accession: NZ_CP038392
• Organism: Escherichia coli O157:H7 strain DEC5B

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A826J9J1
• Locus tag: E3156_RS25985
• Protein ID: WP_064717443.1
• Coordinates: 26469..26618 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	26663..26722 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3156_RS25955	22330..22587	-	258	WP_001418855.1	ribbon-helix-helix domain-containing protein	-
E3156_RS25960	23648..24505	-	858	WP_000131019.1	incFII family plasmid replication initiator RepA	-
E3156_RS25965	24498..24572	-	75	WP_001365705.1	RepA leader peptide Tap	-
E3156_RS25970	24805..25062	-	258	WP_000078114.1	replication regulatory protein RepA	-
E3156_RS25975	25372..25854	-	483	WP_000405244.1	GNAT family N-acetyltransferase	-
E3156_RS25980	25845..26147	-	303	WP_001346256.1	DUF1778 domain-containing protein	-
E3156_RS25985	26469..26618	-	150	WP_064717443.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	26663..26722	+	60	NuclAT_0	-	Antitoxin
-	26663..26722	+	60	NuclAT_0	-	Antitoxin
-	26663..26722	+	60	NuclAT_0	-	Antitoxin
-	26663..26722	+	60	NuclAT_0	-	Antitoxin
E3156_RS25990	26981..27112	-	132	WP_001418856.1	hypothetical protein	-
E3156_RS25995	27297..27509	-	213	WP_032320915.1	hypothetical protein	-
E3156_RS26000	27639..28199	-	561	WP_096837425.1	fertility inhibition protein FinO	-
E3156_RS26005	28254..28994	-	741	WP_000206157.1	conjugal transfer pilus acetylase TraX	-
E3156_RS26010	29014..30720	-	1707	Protein_41	conjugative transfer relaxase/helicase TraI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	espT / espC	1..75328	75328

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5528.64 Da
• Isoelectric Point: 8.7678

>T122312 WP_064717443.1 NZ_CP038392:c26618-26469 [Escherichia coli O157:H7]
MTKYALIGLVAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T122312 NZ_CP038392:c26618-26469 [Escherichia coli O157:H7]
ATGACGAAATATGCCCTTATTGGGTTGGTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT122312 NZ_CP038392:26663-26722 [Escherichia coli O157:H7]
TAAGTACATCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A826J9J1

Antitoxin