Detailed information of TA system TA122306

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4271937..4272631
• Replicon: chromosome
• Accession: NZ_CP038389
• Organism: Escherichia coli O157:H7 strain DEC5B

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: E3156_RS21100
• Protein ID: WP_001263495.1
• Coordinates: 4271937..4272335 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: E3156_RS21105
• Protein ID: WP_000554757.1
• Coordinates: 4272338..4272631 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4267524..4267604	-	81	NuclAT_10	-	-
-	4267524..4267604	-	81	NuclAT_10	-	-
-	4267524..4267604	-	81	NuclAT_10	-	-
-	4267524..4267604	-	81	NuclAT_10	-	-
E3156_RS21075	4268200..4268658	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
E3156_RS21080	4268919..4270376	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
E3156_RS21085	4270433..4270990	-	558	WP_050544425.1	peptide chain release factor H	-
E3156_RS21090	4270902..4271168	-	267	WP_001303804.1	hypothetical protein	-
E3156_RS21095	4271475..4271927	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
E3156_RS21100	4271937..4272335	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
E3156_RS21105	4272338..4272631	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
E3156_RS21110	4272683..4273738	-	1056	WP_001226186.1	DNA polymerase IV	-
E3156_RS21115	4273809..4274594	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
E3156_RS21120	4274539..4276278	+	1740	WP_001301901.1	flagellar biosynthesis protein FlhA	-
E3156_RS21125	4276511..4277008	-	498	Protein_4139	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T122306 WP_001263495.1 NZ_CP038389:c4272335-4271937 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T122306 NZ_CP038389:c4272335-4271937 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT122306 WP_000554757.1 NZ_CP038389:c4272631-4272338 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT122306 NZ_CP038389:c4272631-4272338 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6