Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2011378..2011880 Replicon chromosome
Accession NZ_CP038389
Organism Escherichia coli O157:H7 strain DEC5B

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag E3156_RS09655 Protein ID WP_000767829.1
Coordinates 2011626..2011880 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag E3156_RS09650 Protein ID WP_001259255.1
Coordinates 2011378..2011629 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3156_RS09625 2006555..2007622 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
E3156_RS09630 2007622..2008692 - 1071 WP_000109000.1 histidinol-phosphate transaminase -
E3156_RS09635 2008689..2009993 - 1305 WP_001359325.1 histidinol dehydrogenase -
E3156_RS09640 2009999..2010898 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
E3156_RS09645 2011044..2011094 - 51 WP_013009236.1 his operon leader peptide -
E3156_RS09650 2011378..2011629 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
E3156_RS09655 2011626..2011880 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
E3156_RS09660 2011963..2012787 + 825 WP_000754737.1 SDR family oxidoreductase -
E3156_RS09665 2012833..2013762 + 930 WP_000803334.1 LysR family transcriptional regulator -
E3156_RS09670 2013977..2014039 + 63 WP_010723108.1 membrane protein YoeI -
E3156_RS09675 2014029..2015387 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
E3156_RS09680 2015566..2016624 + 1059 WP_000492327.1 transport protein YeeE -
E3156_RS09685 2016638..2016865 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T122292 WP_000767829.1 NZ_CP038389:2011626-2011880 [Escherichia coli O157:H7]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T122292 NZ_CP038389:2011626-2011880 [Escherichia coli O157:H7]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT122292 WP_001259255.1 NZ_CP038389:2011378-2011629 [Escherichia coli O157:H7]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT122292 NZ_CP038389:2011378-2011629 [Escherichia coli O157:H7]
ATGCGTACAATTAGCTACAGCGAAGCGCGCCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References