Detailed information of TA system TA122279

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 4109587..4110128
• Replicon: chromosome
• Accession: NZ_CP038386
• Organism: Escherichia coli O157:H7 strain DEC5D

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: S1FHQ9
• Locus tag: E3157_RS19935
• Protein ID: WP_000615979.1
• Coordinates: 4109850..4110128 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: C3TP07
• Locus tag: E3157_RS19930
• Protein ID: WP_000729705.1
• Coordinates: 4109587..4109847 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3157_RS19895	4104712..4105110	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	-
E3157_RS19900	4105113..4105406	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	-
E3157_RS19905	4105458..4106513	-	1056	WP_001226186.1	DNA polymerase IV	-
E3157_RS19910	4106584..4107369	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
E3157_RS19915	4107314..4107658	+	345	Protein_3902	FHIPEP family type III secretion protein	-
E3157_RS19920	4107955..4108452	-	498	Protein_3903	REP-associated tyrosine transposase RayT	-
E3157_RS19925	4108628..4109401	-	774	WP_000543891.1	C40 family peptidase	-
E3157_RS19930	4109587..4109847	+	261	WP_000729705.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
E3157_RS19935	4109850..4110128	+	279	WP_000615979.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
E3157_RS19940	4110284..4111024	+	741	WP_001225679.1	murein L,D-transpeptidase	-
E3157_RS19945	4110995..4111762	-	768	WP_001301698.1	class II glutamine amidotransferase	-
E3157_RS19950	4111867..4112445	-	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10830.62 Da
• Isoelectric Point: 10.0702

>T122279 WP_000615979.1 NZ_CP038386:4109850-4110128 [Escherichia coli O157:H7]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T122279 NZ_CP038386:4109850-4110128 [Escherichia coli O157:H7]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9377.74 Da
• Isoelectric Point: 5.0758

>AT122279 WP_000729705.1 NZ_CP038386:4109587-4109847 [Escherichia coli O157:H7]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Nucleotide

• Length: 261 bp

>AT122279 NZ_CP038386:4109587-4109847 [Escherichia coli O157:H7]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA

Structures

Toxin

• AlphaFold DB: S1FHQ9