Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1969663..1970165 Replicon chromosome
Accession NZ_CP038386
Organism Escherichia coli O157:H7 strain DEC5D

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag E3157_RS09295 Protein ID WP_000767829.1
Coordinates 1969911..1970165 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag E3157_RS09290 Protein ID WP_001259255.1
Coordinates 1969663..1969914 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3157_RS09265 1964840..1965907 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
E3157_RS09270 1965907..1966977 - 1071 WP_000109000.1 histidinol-phosphate transaminase -
E3157_RS09275 1966974..1968278 - 1305 WP_001419115.1 histidinol dehydrogenase -
E3157_RS09280 1968284..1969183 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
E3157_RS09285 1969329..1969379 - 51 WP_013009236.1 his operon leader peptide -
E3157_RS09290 1969663..1969914 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
E3157_RS09295 1969911..1970165 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
E3157_RS09300 1970248..1971072 + 825 WP_000754737.1 SDR family oxidoreductase -
E3157_RS09305 1971118..1972047 + 930 WP_000803334.1 LysR family transcriptional regulator -
E3157_RS09310 1972262..1972324 + 63 WP_010723108.1 membrane protein YoeI -
E3157_RS09315 1972314..1973672 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
E3157_RS09320 1973851..1974909 + 1059 WP_000492327.1 transport protein YeeE -
E3157_RS09325 1974923..1975150 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T122265 WP_000767829.1 NZ_CP038386:1969911-1970165 [Escherichia coli O157:H7]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T122265 NZ_CP038386:1969911-1970165 [Escherichia coli O157:H7]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT122265 WP_001259255.1 NZ_CP038386:1969663-1969914 [Escherichia coli O157:H7]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT122265 NZ_CP038386:1969663-1969914 [Escherichia coli O157:H7]
ATGCGTACAATTAGCTACAGCGAAGCGCGCCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References