Detailed information of TA system TA122255

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 41679..41948
• Replicon: plasmid pDEC5E-2
• Accession: NZ_CP038385
• Organism: Escherichia coli O157:H7 strain DEC5E

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: E3158_RS27910
• Protein ID: WP_001312861.1
• Coordinates: 41790..41948 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	41679..41744 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3158_RS27870	36906..37130	-	225	WP_001427866.1	hypothetical protein	-
E3158_RS27875	37053..37244	+	192	WP_032140903.1	hypothetical protein	-
E3158_RS27880	37472..37999	+	528	WP_000290793.1	single-stranded DNA-binding protein	-
E3158_RS27885	38055..38288	+	234	WP_000006004.1	DUF905 domain-containing protein	-
E3158_RS27890	38347..40305	+	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
E3158_RS27895	40360..40794	+	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
E3158_RS27900	40791..41510	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
E3158_RS27905	41522..41710	-	189	WP_001299721.1	hypothetical protein	-
-	41522..41746	+	225	NuclAT_0	-	-
-	41522..41746	+	225	NuclAT_0	-	-
-	41522..41746	+	225	NuclAT_0	-	-
-	41522..41746	+	225	NuclAT_0	-	-
-	41679..41744	+	66	-	-	Antitoxin
E3158_RS27910	41790..41948	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
E3158_RS27915	42302..42526	-	225	WP_001340580.1	hypothetical protein	-
E3158_RS27920	42449..42640	+	192	WP_032140903.1	hypothetical protein	-
E3158_RS27925	42867..43154	+	288	WP_000107537.1	hypothetical protein	-
E3158_RS27930	43275..44096	+	822	WP_001234469.1	DUF945 domain-containing protein	-
E3158_RS27935	44393..45040	-	648	WP_000614282.1	transglycosylase SLT domain-containing protein	-
E3158_RS27940	45326..45709	+	384	WP_001151564.1	relaxosome protein TraM	-
E3158_RS27945	45903..46589	+	687	WP_000332487.1	PAS domain-containing protein	-
E3158_RS27950	46683..46910	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / ant(3'')-Ia / catA1	-	1..80565	80565

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T122255 WP_001312861.1 NZ_CP038385:41790-41948 [Escherichia coli O157:H7]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T122255 NZ_CP038385:41790-41948 [Escherichia coli O157:H7]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT122255 NZ_CP038385:41679-41744 [Escherichia coli O157:H7]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin