Detailed information of TA system TA122246

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 4445381..4445922
• Replicon: chromosome
• Accession: NZ_CP038383
• Organism: Escherichia coli O157:H7 strain DEC5E

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: S1FHQ9
• Locus tag: E3158_RS21815
• Protein ID: WP_000615979.1
• Coordinates: 4445644..4445922 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: C3TP07
• Locus tag: E3158_RS21810
• Protein ID: WP_000729705.1
• Coordinates: 4445381..4445641 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3158_RS21790	4441047..4441832	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
E3158_RS21795	4441777..4443516	+	1740	WP_001419687.1	flagellar biosynthesis protein FlhA	-
E3158_RS21800	4443749..4444246	-	498	Protein_4276	REP-associated tyrosine transposase RayT	-
E3158_RS21805	4444422..4445195	-	774	WP_000543891.1	C40 family peptidase	-
E3158_RS21810	4445381..4445641	+	261	WP_000729705.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
E3158_RS21815	4445644..4445922	+	279	WP_000615979.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
E3158_RS21820	4446078..4446818	+	741	WP_001225679.1	murein L,D-transpeptidase	-
E3158_RS21825	4446789..4447556	-	768	WP_001301698.1	class II glutamine amidotransferase	-
E3158_RS21830	4447661..4448239	-	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10830.62 Da
• Isoelectric Point: 10.0702

>T122246 WP_000615979.1 NZ_CP038383:4445644-4445922 [Escherichia coli O157:H7]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T122246 NZ_CP038383:4445644-4445922 [Escherichia coli O157:H7]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9377.74 Da
• Isoelectric Point: 5.0758

>AT122246 WP_000729705.1 NZ_CP038383:4445381-4445641 [Escherichia coli O157:H7]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Nucleotide

• Length: 261 bp

>AT122246 NZ_CP038383:4445381-4445641 [Escherichia coli O157:H7]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA

Structures

Toxin

• AlphaFold DB: S1FHQ9