Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2085029..2085531 Replicon chromosome
Accession NZ_CP038383
Organism Escherichia coli O157:H7 strain DEC5E

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag E3158_RS10115 Protein ID WP_000767829.1
Coordinates 2085277..2085531 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag E3158_RS10110 Protein ID WP_001259255.1
Coordinates 2085029..2085280 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3158_RS10085 2080206..2081273 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
E3158_RS10090 2081273..2082343 - 1071 WP_000109000.1 histidinol-phosphate transaminase -
E3158_RS10095 2082340..2083644 - 1305 WP_001419115.1 histidinol dehydrogenase -
E3158_RS10100 2083650..2084549 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
E3158_RS10105 2084695..2084745 - 51 WP_013009236.1 his operon leader peptide -
E3158_RS10110 2085029..2085280 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
E3158_RS10115 2085277..2085531 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
E3158_RS10120 2085614..2086438 + 825 WP_000754737.1 SDR family oxidoreductase -
E3158_RS10125 2086484..2087413 + 930 WP_000803334.1 LysR family transcriptional regulator -
E3158_RS10130 2087628..2087690 + 63 WP_010723108.1 membrane protein YoeI -
E3158_RS10135 2087680..2089038 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
E3158_RS10140 2089217..2090275 + 1059 WP_000492327.1 transport protein YeeE -
E3158_RS10145 2090289..2090516 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T122230 WP_000767829.1 NZ_CP038383:2085277-2085531 [Escherichia coli O157:H7]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T122230 NZ_CP038383:2085277-2085531 [Escherichia coli O157:H7]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT122230 WP_001259255.1 NZ_CP038383:2085029-2085280 [Escherichia coli O157:H7]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT122230 NZ_CP038383:2085029-2085280 [Escherichia coli O157:H7]
ATGCGTACAATTAGCTACAGCGAAGCGCGCCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References