Detailed information of TA system TA122225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 704681..705504
• Replicon: chromosome
• Accession: NZ_CP038383
• Organism: Escherichia coli O157:H7 strain DEC5E

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: E3158_RS03440
• Protein ID: WP_000347273.1
• Coordinates: 704681..705145 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: E3158_RS03445
• Protein ID: WP_071781893.1
• Coordinates: 705145..705504 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3158_RS03410	699682..700116	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
E3158_RS03415	700134..701012	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
E3158_RS03420	701002..701781	-	780	WP_000406210.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
E3158_RS03425	701792..702265	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
E3158_RS03430	702288..703568	-	1281	WP_000681946.1	tagatose-bisphosphate aldolase subunit KbaZ	-
E3158_RS03435	703817..704626	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
E3158_RS03440	704681..705145	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
E3158_RS03445	705145..705504	-	360	WP_071781893.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
E3158_RS03450	705501..707045	-	1545	WP_000287615.1	IS3 family transposase	-
E3158_RS03455	707245..708816	-	1572	WP_001273856.1	galactarate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (22-118)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T122225 WP_000347273.1 NZ_CP038383:c705145-704681 [Escherichia coli O157:H7]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T122225 NZ_CP038383:c705145-704681 [Escherichia coli O157:H7]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13425.12 Da
• Isoelectric Point: 6.4820

>AT122225 WP_071781893.1 NZ_CP038383:c705504-705145 [Escherichia coli O157:H7]
INRPRNRPHPPNARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRF
LDTDIQNNPQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 360 bp

>AT122225 NZ_CP038383:c705504-705145 [Escherichia coli O157:H7]
ATAAACCGTCCAAGAAATCGTCCGCACCCCCCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGAT
ACGCGGACAAACAACTATCCCCGCGCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAA
TTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTT
CTGGATACAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCAT
GGACGTCAACATTGATGATGAGATTGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7