Detailed information of TA system TA122213

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 4433387..4433926
• Replicon: chromosome
• Accession: NZ_CP038380
• Organism: Escherichia coli O157:H7 strain E32511

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: E3170_RS22310
• Protein ID: WP_001303998.1
• Coordinates: 4433666..4433926 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: C3TP07
• Locus tag: E3170_RS22305
• Protein ID: WP_000729705.1
• Coordinates: 4433387..4433647 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3170_RS22285	4429141..4429926	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
E3170_RS22290	4429871..4431610	+	1740	WP_001301901.1	flagellar biosynthesis protein FlhA	-
E3170_RS22295	4431755..4432252	-	498	Protein_4367	REP-associated tyrosine transposase RayT	-
E3170_RS22300	4432428..4433201	-	774	WP_000543891.1	C40 family peptidase	-
E3170_RS22305	4433387..4433647	+	261	WP_000729705.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
E3170_RS22310	4433666..4433926	+	261	WP_001303998.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
E3170_RS22315	4434082..4434822	+	741	WP_001225679.1	murein L,D-transpeptidase	-
E3170_RS22320	4434793..4435560	-	768	WP_001301698.1	class II glutamine amidotransferase	-
E3170_RS22325	4435665..4436243	-	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-85)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10026.71 Da
• Isoelectric Point: 10.3188

>T122213 WP_001303998.1 NZ_CP038380:4433666-4433926 [Escherichia coli O157:H7]
MNSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGT
HAALFG

Nucleotide

• Length: 261 bp

>T122213 NZ_CP038380:4433666-4433926 [Escherichia coli O157:H7]
TTGAACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAATAAATTGAAATATCTTAT
GACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAGGTTCATGGAAAGGTTATC
GCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGATTTGAGAGAACTGGAACT
CACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9377.74 Da
• Isoelectric Point: 5.0758

>AT122213 WP_000729705.1 NZ_CP038380:4433387-4433647 [Escherichia coli O157:H7]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Nucleotide

• Length: 261 bp

>AT122213 NZ_CP038380:4433387-4433647 [Escherichia coli O157:H7]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA