Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4427269..4427963 Replicon chromosome
Accession NZ_CP038380
Organism Escherichia coli O157:H7 strain E32511

Toxin (Protein)


Gene name yafO Uniprot ID C3TNX7
Locus tag E3170_RS22270 Protein ID WP_001263495.1
Coordinates 4427269..4427667 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag E3170_RS22275 Protein ID WP_000554757.1
Coordinates 4427670..4427963 (-) Length 98 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 4422856..4422936 - 81 NuclAT_11 - -
- 4422856..4422936 - 81 NuclAT_11 - -
- 4422856..4422936 - 81 NuclAT_11 - -
- 4422856..4422936 - 81 NuclAT_11 - -
E3170_RS22245 4423532..4423990 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
E3170_RS22250 4424251..4425708 + 1458 WP_001293020.1 cytosol nonspecific dipeptidase -
E3170_RS22255 4425765..4426322 - 558 WP_023438539.1 peptide chain release factor H -
E3170_RS22260 4426234..4426500 - 267 WP_001303804.1 hypothetical protein -
E3170_RS22265 4426807..4427259 - 453 WP_001059871.1 GNAT family N-acetyltransferase -
E3170_RS22270 4427269..4427667 - 399 WP_001263495.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
E3170_RS22275 4427670..4427963 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
E3170_RS22280 4428015..4429070 - 1056 WP_001226186.1 DNA polymerase IV -
E3170_RS22285 4429141..4429926 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
E3170_RS22290 4429871..4431610 + 1740 WP_001301901.1 flagellar biosynthesis protein FlhA -
E3170_RS22295 4431755..4432252 - 498 Protein_4367 REP-associated tyrosine transposase RayT -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-130)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 133 a.a.        Molecular weight: 15473.86 Da        Isoelectric Point: 8.0949

>T122212 WP_001263495.1 NZ_CP038380:c4427667-4427269 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T122212 NZ_CP038380:c4427667-4427269 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT122212 WP_000554757.1 NZ_CP038380:c4427963-4427670 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT122212 NZ_CP038380:c4427963-4427670 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References