Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3322946..3323741 Replicon chromosome
Accession NZ_CP038380
Organism Escherichia coli O157:H7 strain E32511

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag E3170_RS17020 Protein ID WP_000854914.1
Coordinates 3322946..3323320 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0B0VS41
Locus tag E3170_RS17025 Protein ID WP_001280954.1
Coordinates 3323367..3323741 (-) Length 125 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3170_RS16980 3317950..3318441 - 492 WP_001301587.1 DUF1097 domain-containing protein -
E3170_RS16985 3318543..3319097 - 555 WP_001001917.1 molecular chaperone YcdY -
E3170_RS16990 3319121..3319858 - 738 WP_000283664.1 zinc-binding phosphatase -
E3170_RS16995 3319913..3320851 - 939 WP_000351284.1 glyoxylate/hydroxypyruvate reductase GhrA -
E3170_RS17005 3321322..3322164 - 843 WP_001280481.1 DUF4942 domain-containing protein -
E3170_RS17010 3322249..3322446 - 198 WP_000839282.1 DUF957 domain-containing protein -
E3170_RS17015 3322458..3322949 - 492 WP_000976853.1 hypothetical protein -
E3170_RS17020 3322946..3323320 - 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
E3170_RS17025 3323367..3323741 - 375 WP_001280954.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
E3170_RS17030 3323904..3324125 - 222 WP_001220314.1 DUF987 domain-containing protein -
E3170_RS17035 3324188..3324664 - 477 WP_001186738.1 RadC family protein -
E3170_RS17040 3324680..3325165 - 486 WP_000214398.1 antirestriction protein -
E3170_RS17045 3325256..3326074 - 819 WP_001234682.1 DUF945 domain-containing protein -
E3170_RS17050 3326414..3327484 - 1071 WP_000102669.1 patatin-like phospholipase family protein -
E3170_RS17055 3327481..3328386 - 906 WP_000203541.1 chemotaxis protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - csgB / csgD / csgE / csgF / csgG 3314332..3325165 10833


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(18-117)


Sequences


Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T122208 WP_000854914.1 NZ_CP038380:c3323320-3322946 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T122208 NZ_CP038380:c3323320-3322946 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13718.51 Da        Isoelectric Point: 6.6249

>AT122208 WP_001280954.1 NZ_CP038380:c3323741-3323367 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Download         Length: 375 bp

>AT122208 NZ_CP038380:c3323741-3323367 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5


Antitoxin

Source ID Structure
AlphaFold DB A0A0B0VS41

References