Detailed information of TA system TA122154

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1038254..1038948
• Replicon: chromosome
• Accession: NZ_CP038376
• Organism: Escherichia coli O157:H7 strain F1273

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: E3159_RS04755
• Protein ID: WP_001263495.1
• Coordinates: 1038550..1038948 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: E3159_RS04750
• Protein ID: WP_000554757.1
• Coordinates: 1038254..1038547 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3159_RS04730	1033965..1034462	+	498	Protein_904	REP-associated tyrosine transposase RayT	-
E3159_RS04735	1034607..1036346	-	1740	WP_001301901.1	flagellar biosynthesis protein FlhA	-
E3159_RS04740	1036291..1037076	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
E3159_RS04745	1037147..1038202	+	1056	WP_001226186.1	DNA polymerase IV	-
E3159_RS04750	1038254..1038547	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
E3159_RS04755	1038550..1038948	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
E3159_RS04760	1038958..1039410	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
E3159_RS04765	1039717..1039983	+	267	WP_001303804.1	hypothetical protein	-
E3159_RS04770	1039895..1040452	+	558	WP_023438539.1	peptide chain release factor H	-
E3159_RS04775	1040509..1041966	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
E3159_RS04780	1042227..1042685	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	1043281..1043361	+	81	NuclAT_11	-	-
-	1043281..1043361	+	81	NuclAT_11	-	-
-	1043281..1043361	+	81	NuclAT_11	-	-
-	1043281..1043361	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T122154 WP_001263495.1 NZ_CP038376:1038550-1038948 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T122154 NZ_CP038376:1038550-1038948 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT122154 WP_000554757.1 NZ_CP038376:1038254-1038547 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT122154 NZ_CP038376:1038254-1038547 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6