Detailed information of TA system TA122119

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 1065356..1065897
• Replicon: chromosome
• Accession: NZ_CP038374
• Organism: Escherichia coli O157:H7 strain F3113

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: S1FHQ9
• Locus tag: E3160_RS04875
• Protein ID: WP_000615979.1
• Coordinates: 1065356..1065634 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: C3TP07
• Locus tag: E3160_RS04880
• Protein ID: WP_000729705.1
• Coordinates: 1065637..1065897 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3160_RS04860	1063039..1063617	+	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-
E3160_RS04865	1063722..1064489	+	768	WP_001301698.1	class II glutamine amidotransferase	-
E3160_RS04870	1064460..1065200	-	741	WP_001225679.1	murein L,D-transpeptidase	-
E3160_RS04875	1065356..1065634	-	279	WP_000615979.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
E3160_RS04880	1065637..1065897	-	261	WP_000729705.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
E3160_RS04885	1066083..1066856	+	774	WP_000543891.1	C40 family peptidase	-
E3160_RS04890	1067032..1067529	+	498	Protein_933	REP-associated tyrosine transposase RayT	-
E3160_RS04895	1067674..1069413	-	1740	WP_001358779.1	flagellar biosynthesis protein FlhA	-
E3160_RS04900	1069358..1070143	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10830.62 Da
• Isoelectric Point: 10.0702

>T122119 WP_000615979.1 NZ_CP038374:c1065634-1065356 [Escherichia coli O157:H7]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T122119 NZ_CP038374:c1065634-1065356 [Escherichia coli O157:H7]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9377.74 Da
• Isoelectric Point: 5.0758

>AT122119 WP_000729705.1 NZ_CP038374:c1065897-1065637 [Escherichia coli O157:H7]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Nucleotide

• Length: 261 bp

>AT122119 NZ_CP038374:c1065897-1065637 [Escherichia coli O157:H7]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA

Structures

Toxin

• AlphaFold DB: S1FHQ9