Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3361941..3362736 Replicon chromosome
Accession NZ_CP038366
Organism Escherichia coli O157:H7 strain F6667

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag E4U72_RS17300 Protein ID WP_000854914.1
Coordinates 3361941..3362315 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0B0VS41
Locus tag E4U72_RS17305 Protein ID WP_001280954.1
Coordinates 3362362..3362736 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U72_RS17260 3356945..3357436 - 492 WP_001301587.1 DUF1097 domain-containing protein -
E4U72_RS17265 3357538..3358092 - 555 WP_001001917.1 molecular chaperone YcdY -
E4U72_RS17270 3358116..3358853 - 738 WP_000283664.1 zinc-binding phosphatase -
E4U72_RS17275 3358908..3359846 - 939 WP_000351284.1 glyoxylate/hydroxypyruvate reductase GhrA -
E4U72_RS17285 3360317..3361159 - 843 WP_001280481.1 DUF4942 domain-containing protein -
E4U72_RS17290 3361244..3361441 - 198 WP_000839282.1 DUF957 domain-containing protein -
E4U72_RS17295 3361453..3361944 - 492 WP_000976853.1 hypothetical protein -
E4U72_RS17300 3361941..3362315 - 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
E4U72_RS17305 3362362..3362736 - 375 WP_001280954.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
E4U72_RS17310 3362899..3363120 - 222 WP_001220314.1 DUF987 domain-containing protein -
E4U72_RS17315 3363183..3363659 - 477 WP_001186738.1 RadC family protein -
E4U72_RS17320 3363675..3364160 - 486 WP_000214398.1 antirestriction protein -
E4U72_RS17325 3364251..3365069 - 819 WP_001234682.1 DUF945 domain-containing protein -
E4U72_RS17330 3365409..3366479 - 1071 WP_000102669.1 patatin-like phospholipase family protein -
E4U72_RS17335 3366476..3367381 - 906 WP_000203541.1 chemotaxis protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - csgB / csgD / csgE / csgF / csgG 3353327..3364160 10833


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T122032 WP_000854914.1 NZ_CP038366:c3362315-3361941 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T122032 NZ_CP038366:c3362315-3361941 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13718.51 Da        Isoelectric Point: 6.6249

>AT122032 WP_001280954.1 NZ_CP038366:c3362736-3362362 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Download         Length: 375 bp

>AT122032 NZ_CP038366:c3362736-3362362 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0VS41

References