Detailed information of TA system TA122029

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2678139..2678364
• Replicon: chromosome
• Accession: NZ_CP038366
• Organism: Escherichia coli O157:H7 strain F6667

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E4U72_RS13675
• Protein ID: WP_000935259.1
• Coordinates: 2678152..2678364 (+)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2678139..2678197 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U72_RS13625	2673204..2673446	+	243	WP_000747948.1	hypothetical protein	-
E4U72_RS13630	2673430..2673855	+	426	WP_000693878.1	Rha family transcriptional regulator	-
E4U72_RS13635	2673924..2674967	+	1044	WP_001262402.1	hypothetical protein	-
E4U72_RS13640	2674960..2675421	+	462	WP_000139447.1	replication protein	-
E4U72_RS13645	2675455..2676171	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
E4U72_RS13650	2676204..2676485	+	282	WP_000603384.1	DNA-binding protein	-
E4U72_RS13655	2676482..2676709	+	228	WP_000699809.1	hypothetical protein	-
E4U72_RS13660	2676702..2677013	+	312	WP_001289673.1	hypothetical protein	-
E4U72_RS13665	2677141..2677359	+	219	WP_000683609.1	DUF4014 family protein	-
E4U72_RS13670	2677361..2677918	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2678139..2678197	-	59	-	-	Antitoxin
E4U72_RS13675	2678152..2678364	+	213	WP_000935259.1	type I toxin-antitoxin system Hok family toxin	Toxin
E4U72_RS13680	2678484..2678828	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
E4U72_RS13685	2678950..2679222	+	273	WP_000191872.1	hypothetical protein	-
E4U72_RS13690	2679224..2680273	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
E4U72_RS13695	2680286..2680591	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
E4U72_RS13700	2680654..2681208	+	555	WP_000640035.1	DUF1133 family protein	-
E4U72_RS13705	2681433..2681630	+	198	WP_000917763.1	hypothetical protein	-
E4U72_RS13710	2681766..2682479	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
E4U72_RS13725	2682930..2683361	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2605952..2733175	127223

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7887.47 Da
• Isoelectric Point: 9.2654

>T122029 WP_000935259.1 NZ_CP038366:2678152-2678364 [Escherichia coli O157:H7]
MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 213 bp

>T122029 NZ_CP038366:2678152-2678364 [Escherichia coli O157:H7]
ATGCTGAACACATGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTGCGAATCC
GAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT122029 NZ_CP038366:c2678197-2678139 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Antitoxin