Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4488461..4489155 Replicon chromosome
Accession NZ_CP038363
Organism Escherichia coli O157:H7 strain F7349

Toxin (Protein)


Gene name yafO Uniprot ID C3TNX7
Locus tag E4U70_RS22550 Protein ID WP_001263495.1
Coordinates 4488461..4488859 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag E4U70_RS22555 Protein ID WP_000554757.1
Coordinates 4488862..4489155 (-) Length 98 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 4484048..4484128 - 81 NuclAT_12 - -
- 4484048..4484128 - 81 NuclAT_12 - -
- 4484048..4484128 - 81 NuclAT_12 - -
- 4484048..4484128 - 81 NuclAT_12 - -
E4U70_RS22525 4484724..4485182 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
E4U70_RS22530 4485443..4486900 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
E4U70_RS22535 4486957..4487514 - 558 WP_023438539.1 peptide chain release factor H -
E4U70_RS22540 4487426..4487692 - 267 WP_001303804.1 hypothetical protein -
E4U70_RS22545 4487999..4488451 - 453 WP_001059871.1 GNAT family N-acetyltransferase -
E4U70_RS22550 4488461..4488859 - 399 WP_001263495.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
E4U70_RS22555 4488862..4489155 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
E4U70_RS22560 4489207..4490262 - 1056 WP_001226188.1 DNA polymerase IV -
E4U70_RS22565 4490333..4491118 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
E4U70_RS22570 4491063..4492802 + 1740 WP_001301901.1 flagellar biosynthesis protein FlhA -
E4U70_RS22575 4492947..4493444 - 498 Protein_4425 REP-associated tyrosine transposase RayT -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4451620..4494393 42773


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-130)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 133 a.a.        Molecular weight: 15473.86 Da        Isoelectric Point: 8.0949

>T122002 WP_001263495.1 NZ_CP038363:c4488859-4488461 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T122002 NZ_CP038363:c4488859-4488461 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT122002 WP_000554757.1 NZ_CP038363:c4489155-4488862 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT122002 NZ_CP038363:c4489155-4488862 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References