Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4366495..4367034 Replicon chromosome
Accession NZ_CP038360
Organism Escherichia coli O157:H7 strain F7386

Toxin (Protein)


Gene name yafQ Uniprot ID -
Locus tag E4U67_RS21935 Protein ID WP_001303998.1
Coordinates 4366774..4367034 (+) Length 87 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID C3TP07
Locus tag E4U67_RS21930 Protein ID WP_000729705.1
Coordinates 4366495..4366755 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U67_RS21910 4362249..4363034 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
E4U67_RS21915 4362979..4364718 + 1740 WP_001301901.1 flagellar biosynthesis protein FlhA -
E4U67_RS21920 4364863..4365360 - 498 Protein_4291 REP-associated tyrosine transposase RayT -
E4U67_RS21925 4365536..4366309 - 774 WP_000543891.1 C40 family peptidase -
E4U67_RS21930 4366495..4366755 + 261 WP_000729705.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
E4U67_RS21935 4366774..4367034 + 261 WP_001303998.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
E4U67_RS21940 4367190..4367930 + 741 WP_001225679.1 murein L,D-transpeptidase -
E4U67_RS21945 4367901..4368668 - 768 WP_001301698.1 class II glutamine amidotransferase -
E4U67_RS21950 4368773..4369351 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-85)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 87 a.a.        Molecular weight: 10026.71 Da        Isoelectric Point: 10.3188

>T121966 WP_001303998.1 NZ_CP038360:4366774-4367034 [Escherichia coli O157:H7]
MNSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGT
HAALFG

Download         Length: 261 bp

>T121966 NZ_CP038360:4366774-4367034 [Escherichia coli O157:H7]
TTGAACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAATAAATTGAAATATCTTAT
GACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAGGTTCATGGAAAGGTTATC
GCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGATTTGAGAGAACTGGAACT
CACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9377.74 Da        Isoelectric Point: 5.0758

>AT121966 WP_000729705.1 NZ_CP038360:4366495-4366755 [Escherichia coli O157:H7]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Download         Length: 261 bp

>AT121966 NZ_CP038360:4366495-4366755 [Escherichia coli O157:H7]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References