Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3253407..3254202 Replicon chromosome
Accession NZ_CP038360
Organism Escherichia coli O157:H7 strain F7386

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag E4U67_RS16635 Protein ID WP_000854914.1
Coordinates 3253407..3253781 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0B0VS41
Locus tag E4U67_RS16640 Protein ID WP_001280954.1
Coordinates 3253828..3254202 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U67_RS16595 3248411..3248902 - 492 WP_001301587.1 DUF1097 domain-containing protein -
E4U67_RS16600 3249004..3249558 - 555 WP_001001917.1 molecular chaperone YcdY -
E4U67_RS16605 3249582..3250319 - 738 WP_000283664.1 zinc-binding phosphatase -
E4U67_RS16610 3250374..3251312 - 939 WP_000351284.1 glyoxylate/hydroxypyruvate reductase GhrA -
E4U67_RS16620 3251783..3252625 - 843 WP_001280481.1 DUF4942 domain-containing protein -
E4U67_RS16625 3252710..3252907 - 198 WP_000839282.1 DUF957 domain-containing protein -
E4U67_RS16630 3252919..3253410 - 492 WP_000976853.1 hypothetical protein -
E4U67_RS16635 3253407..3253781 - 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
E4U67_RS16640 3253828..3254202 - 375 WP_001280954.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
E4U67_RS16645 3254365..3254586 - 222 WP_001220314.1 DUF987 domain-containing protein -
E4U67_RS16650 3254649..3255125 - 477 WP_001186738.1 RadC family protein -
E4U67_RS16655 3255141..3255626 - 486 WP_000214398.1 antirestriction protein -
E4U67_RS16660 3255717..3256535 - 819 WP_001234682.1 DUF945 domain-containing protein -
E4U67_RS16665 3256875..3257945 - 1071 WP_000102669.1 patatin-like phospholipase family protein -
E4U67_RS16670 3257942..3258847 - 906 WP_000203541.1 chemotaxis protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - csgB / csgD / csgE / csgF / csgG 3244793..3255626 10833


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T121959 WP_000854914.1 NZ_CP038360:c3253781-3253407 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T121959 NZ_CP038360:c3253781-3253407 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13718.51 Da        Isoelectric Point: 6.6249

>AT121959 WP_001280954.1 NZ_CP038360:c3254202-3253828 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Download         Length: 375 bp

>AT121959 NZ_CP038360:c3254202-3253828 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0VS41

References