Detailed information of TA system TA121956

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2593862..2594087
• Replicon: chromosome
• Accession: NZ_CP038360
• Organism: Escherichia coli O157:H7 strain F7386

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E4U67_RS13110
• Protein ID: WP_000935259.1
• Coordinates: 2593875..2594087 (+)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2593862..2593920 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U67_RS13060	2588927..2589169	+	243	WP_000747948.1	hypothetical protein	-
E4U67_RS13065	2589153..2589578	+	426	WP_000693878.1	Rha family transcriptional regulator	-
E4U67_RS13070	2589647..2590690	+	1044	WP_001262402.1	hypothetical protein	-
E4U67_RS13075	2590683..2591144	+	462	WP_000139447.1	replication protein	-
E4U67_RS13080	2591178..2591894	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
E4U67_RS13085	2591927..2592208	+	282	WP_000603384.1	DNA-binding protein	-
E4U67_RS13090	2592205..2592432	+	228	WP_000699808.1	hypothetical protein	-
E4U67_RS13095	2592425..2592736	+	312	WP_001289673.1	hypothetical protein	-
E4U67_RS13100	2592864..2593082	+	219	WP_000683609.1	DUF4014 family protein	-
E4U67_RS13105	2593084..2593641	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2593862..2593920	-	59	-	-	Antitoxin
E4U67_RS13110	2593875..2594087	+	213	WP_000935259.1	type I toxin-antitoxin system Hok family toxin	Toxin
E4U67_RS13115	2594207..2594551	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
E4U67_RS13120	2594673..2594945	+	273	WP_000191872.1	hypothetical protein	-
E4U67_RS13125	2594947..2595996	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
E4U67_RS13130	2596009..2596314	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
E4U67_RS13135	2596377..2596931	+	555	WP_000640035.1	DUF1133 family protein	-
E4U67_RS13140	2597156..2597353	+	198	WP_000917763.1	hypothetical protein	-
E4U67_RS13145	2597489..2598202	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
E4U67_RS13160	2598653..2599084	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2525476..2624538	99062

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7887.47 Da
• Isoelectric Point: 9.2654

>T121956 WP_000935259.1 NZ_CP038360:2593875-2594087 [Escherichia coli O157:H7]
MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 213 bp

>T121956 NZ_CP038360:2593875-2594087 [Escherichia coli O157:H7]
ATGCTGAACACATGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTGCGAATCC
GAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT121956 NZ_CP038360:c2593920-2593862 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Antitoxin