Detailed information of TA system TA121891

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3526810..3527035
• Replicon: chromosome
• Accession: NZ_CP038355
• Organism: Escherichia coli O157:H7 str. F8092B

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E4U83_RS18075
• Protein ID: WP_000813263.1
• Coordinates: 3526810..3526965 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3526977..3527035 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U83_RS18040	3522264..3522977	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
E4U83_RS18045	3523115..3523311	-	197	Protein_3533	TrmB family transcriptional regulator	-
E4U83_RS18050	3523598..3524416	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
E4U83_RS18055	3524568..3524939	-	372	WP_000090264.1	antitermination protein	-
E4U83_RS18060	3524929..3525300	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
E4U83_RS18065	3525313..3526362	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
E4U83_RS18070	3526364..3526642	-	279	WP_001341388.1	hypothetical protein	-
E4U83_RS18075	3526810..3526965	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3526977..3527035	+	59	-	-	Antitoxin
E4U83_RS18080	3527067..3527204	+	138	WP_000955173.1	hypothetical protein	-
E4U83_RS18085	3527570..3528343	+	774	WP_000160654.1	alpha/beta hydrolase	-
E4U83_RS18090	3528695..3529108	-	414	WP_001151233.1	DUF977 family protein	-
E4U83_RS18095	3529124..3529894	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
E4U83_RS18100	3529916..3530662	-	747	WP_000788751.1	ATP-binding protein	-
E4U83_RS18105	3530669..3531760	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3492299..3541423	49124

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T121891 WP_000813263.1 NZ_CP038355:c3526965-3526810 [Escherichia coli O157:H7 str. F8092B]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T121891 NZ_CP038355:c3526965-3526810 [Escherichia coli O157:H7 str. F8092B]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT121891 NZ_CP038355:3526977-3527035 [Escherichia coli O157:H7 str. F8092B]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin