Detailed information of TA system TA121886

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2912235..2912460
• Replicon: chromosome
• Accession: NZ_CP038355
• Organism: Escherichia coli O157:H7 str. F8092B

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E4U83_RS14550
• Protein ID: WP_000935259.1
• Coordinates: 2912235..2912447 (-)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2912402..2912460 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U83_RS14500	2907238..2907669	-	432	WP_001302123.1	tellurite resistance protein	-
E4U83_RS14515	2908120..2908833	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
E4U83_RS14520	2908969..2909166	-	198	WP_000917763.1	hypothetical protein	-
E4U83_RS14525	2909391..2909945	-	555	WP_000640035.1	DUF1133 family protein	-
E4U83_RS14530	2910008..2910313	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
E4U83_RS14535	2910326..2911375	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
E4U83_RS14540	2911377..2911649	-	273	WP_000191872.1	hypothetical protein	-
E4U83_RS14545	2911771..2912115	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
E4U83_RS14550	2912235..2912447	-	213	WP_000935259.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2912402..2912460	+	59	-	-	Antitoxin
E4U83_RS14555	2912681..2913238	-	558	WP_000104474.1	DUF551 domain-containing protein	-
E4U83_RS14560	2913240..2913458	-	219	WP_000683609.1	DUF4014 family protein	-
E4U83_RS14565	2913586..2913897	-	312	WP_001289673.1	hypothetical protein	-
E4U83_RS14570	2913890..2914117	-	228	WP_000699809.1	hypothetical protein	-
E4U83_RS14575	2914114..2914395	-	282	WP_000603384.1	DNA-binding protein	-
E4U83_RS14580	2914428..2915144	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
E4U83_RS14585	2915178..2915639	-	462	WP_000139447.1	replication protein	-
E4U83_RS14590	2915632..2916675	-	1044	WP_001262402.1	hypothetical protein	-
E4U83_RS14595	2916744..2917169	-	426	WP_000693878.1	Rha family transcriptional regulator	-
E4U83_RS14600	2917153..2917395	-	243	WP_000747948.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7' / espM1 / nleA/espI / paa	2875121..3034105	158984

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7887.47 Da
• Isoelectric Point: 9.2654

>T121886 WP_000935259.1 NZ_CP038355:c2912447-2912235 [Escherichia coli O157:H7 str. F8092B]
MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 213 bp

>T121886 NZ_CP038355:c2912447-2912235 [Escherichia coli O157:H7 str. F8092B]
ATGCTGAACACATGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTGCGAATCC
GAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT121886 NZ_CP038355:2912402-2912460 [Escherichia coli O157:H7 str. F8092B]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Antitoxin