Detailed information of TA system TA121850

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2673517..2673742
• Replicon: chromosome
• Accession: NZ_CP038353
• Organism: Escherichia coli O157:H7 strain F8797

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E4U81_RS13680
• Protein ID: WP_000935259.1
• Coordinates: 2673530..2673742 (+)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2673517..2673575 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U81_RS13630	2668582..2668824	+	243	WP_000747948.1	hypothetical protein	-
E4U81_RS13635	2668808..2669233	+	426	WP_000693878.1	Rha family transcriptional regulator	-
E4U81_RS13640	2669302..2670345	+	1044	WP_001262402.1	hypothetical protein	-
E4U81_RS13645	2670338..2670799	+	462	WP_000139447.1	replication protein	-
E4U81_RS13650	2670833..2671549	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
E4U81_RS13655	2671582..2671863	+	282	WP_000603384.1	DNA-binding protein	-
E4U81_RS13660	2671860..2672087	+	228	WP_000699809.1	hypothetical protein	-
E4U81_RS13665	2672080..2672391	+	312	WP_001289673.1	hypothetical protein	-
E4U81_RS13670	2672519..2672737	+	219	WP_000683609.1	DUF4014 family protein	-
E4U81_RS13675	2672739..2673296	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2673517..2673575	-	59	-	-	Antitoxin
E4U81_RS13680	2673530..2673742	+	213	WP_000935259.1	type I toxin-antitoxin system Hok family toxin	Toxin
E4U81_RS13685	2673862..2674206	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
E4U81_RS13690	2674328..2674600	+	273	WP_000191872.1	hypothetical protein	-
E4U81_RS13695	2674602..2675651	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
E4U81_RS13700	2675664..2675969	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
E4U81_RS13705	2676032..2676586	+	555	WP_000640035.1	DUF1133 family protein	-
E4U81_RS13710	2676811..2677008	+	198	WP_000917763.1	hypothetical protein	-
E4U81_RS13715	2677144..2677857	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
E4U81_RS13730	2678308..2678739	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2605130..2704196	99066

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7887.47 Da
• Isoelectric Point: 9.2654

>T121850 WP_000935259.1 NZ_CP038353:2673530-2673742 [Escherichia coli O157:H7]
MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 213 bp

>T121850 NZ_CP038353:2673530-2673742 [Escherichia coli O157:H7]
ATGCTGAACACATGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTGCGAATCC
GAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT121850 NZ_CP038353:c2673575-2673517 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Antitoxin