Detailed information of TA system TA121823

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4528245..4528939
• Replicon: chromosome
• Accession: NZ_CP038351
• Organism: Escherichia coli O157:H7 strain F8798

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: E4U69_RS23055
• Protein ID: WP_001263495.1
• Coordinates: 4528245..4528643 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: E4U69_RS23060
• Protein ID: WP_000554757.1
• Coordinates: 4528646..4528939 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4523832..4523912	-	81	NuclAT_12	-	-
-	4523832..4523912	-	81	NuclAT_12	-	-
-	4523832..4523912	-	81	NuclAT_12	-	-
-	4523832..4523912	-	81	NuclAT_12	-	-
E4U69_RS23030	4524508..4524966	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
E4U69_RS23035	4525227..4526684	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
E4U69_RS23040	4526741..4527298	-	558	WP_023438539.1	peptide chain release factor H	-
E4U69_RS23045	4527210..4527476	-	267	WP_001303804.1	hypothetical protein	-
E4U69_RS23050	4527783..4528235	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
E4U69_RS23055	4528245..4528643	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
E4U69_RS23060	4528646..4528939	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
E4U69_RS23065	4528991..4530046	-	1056	WP_001226186.1	DNA polymerase IV	-
E4U69_RS23070	4530117..4530902	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
E4U69_RS23075	4530847..4532586	+	1740	WP_001301901.1	flagellar biosynthesis protein FlhA	-
E4U69_RS23080	4532731..4533228	-	498	Protein_4520	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4490112..4534177	44065

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T121823 WP_001263495.1 NZ_CP038351:c4528643-4528245 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T121823 NZ_CP038351:c4528643-4528245 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT121823 WP_000554757.1 NZ_CP038351:c4528939-4528646 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT121823 NZ_CP038351:c4528939-4528646 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6