Detailed information of TA system TA121815

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2640445..2640816
• Replicon: chromosome
• Accession: NZ_CP038351
• Organism: Escherichia coli O157:H7 strain F8798

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: E4U69_RS13430
• Protein ID: WP_001443846.1
• Coordinates: 2640667..2640816 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2640445..2640623 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U69_RS13400	2636197..2636367	+	171	WP_001625136.1	hypothetical protein	-
E4U69_RS13405	2636400..2637773	+	1374	WP_000123746.1	ATP-dependent RNA helicase DbpA	-
E4U69_RS13410	2637902..2638837	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
E4U69_RS13415	2638889..2640124	-	1236	WP_000040839.1	site-specific integrase	-
E4U69_RS13420	2640126..2640341	-	216	WP_000079604.1	excisionase XisR	-
E4U69_RS13425	2640441..2640629	-	189	WP_001302840.1	DUF1187 family protein	-
-	2640445..2640623	+	179	NuclAT_0	-	Antitoxin
-	2640445..2640623	+	179	NuclAT_0	-	Antitoxin
-	2640445..2640623	+	179	NuclAT_0	-	Antitoxin
-	2640445..2640623	+	179	NuclAT_0	-	Antitoxin
E4U69_RS13430	2640667..2640816	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
E4U69_RS13435	2640872..2641681	-	810	WP_000166313.1	recombination protein RecT	-
E4U69_RS13440	2641674..2644274	-	2601	WP_000105153.1	exodeoxyribonuclease VIII	-
E4U69_RS13445	2644376..2644651	-	276	WP_001344816.1	hypothetical protein	-
E4U69_RS13450	2644726..2644896	-	171	WP_001352098.1	conserved protein, Rac prophage	-
E4U69_RS13455	2644896..2645117	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2582208..2688203	105995

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T121815 WP_001443846.1 NZ_CP038351:c2640816-2640667 [Escherichia coli O157:H7]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T121815 NZ_CP038351:c2640816-2640667 [Escherichia coli O157:H7]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT121815 NZ_CP038351:2640445-2640623 [Escherichia coli O157:H7]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin