Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3202223..3203018 Replicon chromosome
Accession NZ_CP038349
Organism Escherichia coli O157:H7 strain F8952

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag E4U62_RS16180 Protein ID WP_000854914.1
Coordinates 3202223..3202597 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0B0VS41
Locus tag E4U62_RS16185 Protein ID WP_001280954.1
Coordinates 3202644..3203018 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U62_RS16140 3197227..3197718 - 492 WP_001301587.1 DUF1097 domain-containing protein -
E4U62_RS16145 3197820..3198374 - 555 WP_001001917.1 molecular chaperone YcdY -
E4U62_RS16150 3198398..3199135 - 738 WP_000283664.1 zinc-binding phosphatase -
E4U62_RS16155 3199190..3200128 - 939 WP_000351284.1 glyoxylate/hydroxypyruvate reductase GhrA -
E4U62_RS16165 3200599..3201441 - 843 WP_001280481.1 DUF4942 domain-containing protein -
E4U62_RS16170 3201526..3201723 - 198 WP_000839282.1 DUF957 domain-containing protein -
E4U62_RS16175 3201735..3202226 - 492 WP_000976853.1 hypothetical protein -
E4U62_RS16180 3202223..3202597 - 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
E4U62_RS16185 3202644..3203018 - 375 WP_001280954.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
E4U62_RS16190 3203181..3203402 - 222 WP_001220314.1 DUF987 domain-containing protein -
E4U62_RS16195 3203465..3203941 - 477 WP_001186738.1 RadC family protein -
E4U62_RS16200 3203957..3204442 - 486 WP_000214398.1 antirestriction protein -
E4U62_RS16205 3204533..3205351 - 819 WP_001234682.1 DUF945 domain-containing protein -
E4U62_RS16210 3205691..3206761 - 1071 WP_000102669.1 patatin-like phospholipase family protein -
E4U62_RS16215 3206758..3207663 - 906 WP_000203541.1 chemotaxis protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - csgB / csgD / csgE / csgF / csgG 3193609..3204442 10833


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T121785 WP_000854914.1 NZ_CP038349:c3202597-3202223 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T121785 NZ_CP038349:c3202597-3202223 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13718.51 Da        Isoelectric Point: 6.6249

>AT121785 WP_001280954.1 NZ_CP038349:c3203018-3202644 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Download         Length: 375 bp

>AT121785 NZ_CP038349:c3203018-3202644 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0VS41

References