Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type I Classification (family/domain) ralRA/-
Location 2579438..2579809 Replicon chromosome
Accession NZ_CP038349
Organism Escherichia coli O157:H7 strain F8952

Toxin (Protein)


Gene name ralR Uniprot ID A0A0D6ZRD3
Locus tag E4U62_RS12710 Protein ID WP_001443846.1
Coordinates 2579660..2579809 (-) Length 50 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 2579438..2579616 (+)

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U62_RS12680 2575190..2575360 + 171 WP_001625136.1 hypothetical protein -
E4U62_RS12685 2575393..2576766 + 1374 WP_000123746.1 ATP-dependent RNA helicase DbpA -
E4U62_RS12690 2576895..2577830 - 936 WP_001157407.1 tRNA 2-thiocytidine(32) synthetase TtcA -
E4U62_RS12695 2577882..2579117 - 1236 WP_000040839.1 site-specific integrase -
E4U62_RS12700 2579119..2579334 - 216 WP_000079604.1 excisionase XisR -
E4U62_RS12705 2579434..2579622 - 189 WP_001302840.1 DUF1187 family protein -
- 2579438..2579616 + 179 NuclAT_0 - Antitoxin
- 2579438..2579616 + 179 NuclAT_0 - Antitoxin
- 2579438..2579616 + 179 NuclAT_0 - Antitoxin
- 2579438..2579616 + 179 NuclAT_0 - Antitoxin
E4U62_RS12710 2579660..2579809 - 150 WP_001443846.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
E4U62_RS12715 2579865..2580674 - 810 WP_000166313.1 recombination protein RecT -
E4U62_RS12720 2580667..2583267 - 2601 WP_000105140.1 exodeoxyribonuclease VIII -
E4U62_RS12725 2583369..2583644 - 276 WP_001344816.1 hypothetical protein -
E4U62_RS12730 2583719..2583889 - 171 WP_001352098.1 conserved protein, Rac prophage -
E4U62_RS12735 2583889..2584110 - 222 WP_000560223.1 killing protein KilR -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2565619..2629945 64326


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(6-48)


Sequences


Toxin        


Download         Length: 50 a.a.        Molecular weight: 5383.12 Da        Isoelectric Point: 8.3398

>T121780 WP_001443846.1 NZ_CP038349:c2579809-2579660 [Escherichia coli O157:H7]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Download         Length: 150 bp

>T121780 NZ_CP038349:c2579809-2579660 [Escherichia coli O157:H7]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin


Download         Length: 179 bp

>AT121780 NZ_CP038349:2579438-2579616 [Escherichia coli O157:H7]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0D6ZRD3


Antitoxin

Download structure file

References