Detailed information of TA system TA121757

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 85648..85912
• Replicon: plasmid pG5295-2
• Accession: NZ_CP038347
• Organism: Escherichia coli O157:H7 strain G5295

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: E4U77_RS28130
• Protein ID: WP_001303307.1
• Coordinates: 85648..85800 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	85855..85912 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U77_RS28105	80925..83093	+	2169	WP_171880343.1	DotA/TraY family protein	-
E4U77_RS28110	83167..83817	+	651	WP_001178506.1	plasmid IncI1-type surface exclusion protein ExcA	-
E4U77_RS28115	83889..84098	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
E4U77_RS28120	84490..84666	+	177	WP_001054897.1	hypothetical protein	-
E4U77_RS28125	85325..85576	+	252	WP_001291965.1	hypothetical protein	-
E4U77_RS28130	85648..85800	-	153	WP_001303307.1	Hok/Gef family protein	Toxin
-	85855..85912	+	58	NuclAT_0	-	Antitoxin
-	85855..85912	+	58	NuclAT_0	-	Antitoxin
-	85855..85912	+	58	NuclAT_0	-	Antitoxin
-	85855..85912	+	58	NuclAT_0	-	Antitoxin
E4U77_RS28135	86092..87300	+	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
E4U77_RS28140	87319..88389	+	1071	WP_000151582.1	IncI1-type conjugal transfer protein TrbB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..89068	89068

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T121757 WP_001303307.1 NZ_CP038347:c85800-85648 [Escherichia coli O157:H7]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T121757 NZ_CP038347:c85800-85648 [Escherichia coli O157:H7]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 58 bp

>AT121757 NZ_CP038347:85855-85912 [Escherichia coli O157:H7]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin