Detailed information of TA system TA121704

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2561721..2561946
• Replicon: chromosome
• Accession: NZ_CP038344
• Organism: Escherichia coli O157:H7 strain Gim1-1

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E4U63_RS12835
• Protein ID: WP_000935259.1
• Coordinates: 2561734..2561946 (+)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2561721..2561779 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U63_RS12785	2556786..2557028	+	243	WP_000747948.1	hypothetical protein	-
E4U63_RS12790	2557012..2557437	+	426	WP_000693878.1	Rha family transcriptional regulator	-
E4U63_RS12795	2557506..2558549	+	1044	WP_001262402.1	hypothetical protein	-
E4U63_RS12800	2558542..2559003	+	462	WP_000139447.1	replication protein	-
E4U63_RS12805	2559037..2559753	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
E4U63_RS12810	2559786..2560067	+	282	WP_000603384.1	DNA-binding protein	-
E4U63_RS12815	2560064..2560291	+	228	WP_000699809.1	hypothetical protein	-
E4U63_RS12820	2560284..2560595	+	312	WP_001289673.1	hypothetical protein	-
E4U63_RS12825	2560723..2560941	+	219	WP_000683609.1	DUF4014 family protein	-
E4U63_RS12830	2560943..2561500	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2561721..2561779	-	59	-	-	Antitoxin
E4U63_RS12835	2561734..2561946	+	213	WP_000935259.1	type I toxin-antitoxin system Hok family toxin	Toxin
E4U63_RS12840	2562066..2562410	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
E4U63_RS12845	2562532..2562804	+	273	WP_000191872.1	hypothetical protein	-
E4U63_RS12850	2562806..2563855	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
E4U63_RS12855	2563868..2564173	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
E4U63_RS12860	2564236..2564790	+	555	WP_000640035.1	DUF1133 family protein	-
E4U63_RS12865	2565015..2565212	+	198	WP_000917763.1	hypothetical protein	-
E4U63_RS12870	2565348..2566061	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
E4U63_RS12885	2566516..2566944	+	429	WP_001303509.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2492444..2640552	148108

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7887.47 Da
• Isoelectric Point: 9.2654

>T121704 WP_000935259.1 NZ_CP038344:2561734-2561946 [Escherichia coli O157:H7]
MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 213 bp

>T121704 NZ_CP038344:2561734-2561946 [Escherichia coli O157:H7]
ATGCTGAACACATGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTGCGAATCC
GAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT121704 NZ_CP038344:c2561779-2561721 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Antitoxin