Detailed information of TA system TA121637

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2642332..2642557
• Replicon: chromosome
• Accession: NZ_CP038339
• Organism: Escherichia coli O157:H7 strain H6437

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E3164_RS13415
• Protein ID: WP_000935259.1
• Coordinates: 2642345..2642557 (+)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2642332..2642390 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3164_RS13365	2637384..2637627	+	244	Protein_2611	hypothetical protein	-
E3164_RS13370	2637611..2638036	+	426	WP_000693878.1	Rha family transcriptional regulator	-
E3164_RS13375	2638105..2639160	+	1056	WP_001356791.1	hypothetical protein	-
E3164_RS13380	2639153..2639614	+	462	WP_000139447.1	replication protein	-
E3164_RS13385	2639648..2640364	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
E3164_RS13390	2640397..2640678	+	282	WP_000603384.1	DNA-binding protein	-
E3164_RS13395	2640675..2640902	+	228	WP_000699809.1	hypothetical protein	-
E3164_RS13400	2640895..2641206	+	312	WP_001289673.1	hypothetical protein	-
E3164_RS13405	2641334..2641552	+	219	WP_000683609.1	DUF4014 family protein	-
E3164_RS13410	2641554..2642111	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2642332..2642390	-	59	-	-	Antitoxin
E3164_RS13415	2642345..2642557	+	213	WP_000935259.1	type I toxin-antitoxin system Hok family toxin	Toxin
E3164_RS13420	2642677..2643021	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
E3164_RS13425	2643143..2643415	+	273	WP_000191871.1	hypothetical protein	-
E3164_RS13430	2643417..2644466	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
E3164_RS13435	2644479..2644784	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
E3164_RS13440	2644847..2645401	+	555	WP_000640035.1	DUF1133 family protein	-
E3164_RS13445	2645626..2645823	+	198	WP_000917763.1	hypothetical protein	-
E3164_RS13450	2645959..2646672	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
E3164_RS13465	2647123..2647554	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2575249..2680004	104755

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7887.47 Da
• Isoelectric Point: 9.2654

>T121637 WP_000935259.1 NZ_CP038339:2642345-2642557 [Escherichia coli O157:H7]
MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 213 bp

>T121637 NZ_CP038339:2642345-2642557 [Escherichia coli O157:H7]
ATGCTGAACACATGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTGCGAATCC
GAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT121637 NZ_CP038339:c2642390-2642332 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Antitoxin