Detailed information of TA system TA121614

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 49616..50037
• Replicon: plasmid pLSU61-1
• Accession: NZ_CP038338
• Organism: Escherichia coli O157:H7 strain LSU61

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: E3165_RS27695
• Protein ID: WP_001302184.1
• Coordinates: 49879..50037 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	49616..49814 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3165_RS27660	44882..45331	-	450	WP_001451816.1	hypothetical protein	-
E3165_RS27665	45401..45607	+	207	WP_000547965.1	hypothetical protein	-
E3165_RS27670	45633..46085	+	453	WP_000290823.1	single-stranded DNA-binding protein	-
E3165_RS27675	46142..46375	+	234	WP_000005995.1	DUF905 family protein	-
E3165_RS27680	46441..48399	+	1959	WP_171877392.1	ParB/RepB/Spo0J family partition protein	-
E3165_RS27685	48454..48888	+	435	WP_000845908.1	conjugation system SOS inhibitor PsiB	-
E3165_RS27690	48885..49604	+	720	WP_001276249.1	plasmid SOS inhibition protein A	-
-	49616..49814	+	199	NuclAT_0	-	Antitoxin
-	49616..49814	+	199	NuclAT_0	-	Antitoxin
-	49616..49814	+	199	NuclAT_0	-	Antitoxin
-	49616..49814	+	199	NuclAT_0	-	Antitoxin
E3165_RS27695	49879..50037	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
E3165_RS27700	50275..50592	-	318	WP_000820278.1	hypothetical protein	-
E3165_RS27705	50636..50872	-	237	WP_171877393.1	hypothetical protein	-
E3165_RS27710	51025..51222	+	198	WP_045174704.1	hypothetical protein	-
E3165_RS27715	51341..52162	+	822	WP_001234448.1	DUF945 domain-containing protein	-
E3165_RS27720	52458..53048	-	591	WP_168940513.1	transglycosylase SLT domain-containing protein	-
E3165_RS27725	53399..53782	+	384	WP_001063021.1	relaxosome protein TraM	-
E3165_RS27730	53973..54620	+	648	WP_171877394.1	conjugal transfer transcriptional regulator TraJ	-
E3165_RS27735	54756..54971	+	216	WP_000086381.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / papC	1..119161	119161

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T121614 WP_001302184.1 NZ_CP038338:49879-50037 [Escherichia coli O157:H7]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T121614 NZ_CP038338:49879-50037 [Escherichia coli O157:H7]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATAGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT121614 NZ_CP038338:49616-49814 [Escherichia coli O157:H7]
TCACACGGATTGCCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAATGTGGTCAGCGTGCTGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin