Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4441556..4442250 Replicon chromosome
Accession NZ_CP038336
Organism Escherichia coli O157:H7 strain LSU61

Toxin (Protein)


Gene name yafO Uniprot ID C3TNX7
Locus tag E3165_RS22250 Protein ID WP_001263495.1
Coordinates 4441556..4441954 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag E3165_RS22255 Protein ID WP_000554757.1
Coordinates 4441957..4442250 (-) Length 98 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 4437143..4437223 - 81 NuclAT_11 - -
- 4437143..4437223 - 81 NuclAT_11 - -
- 4437143..4437223 - 81 NuclAT_11 - -
- 4437143..4437223 - 81 NuclAT_11 - -
E3165_RS22225 4437819..4438277 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
E3165_RS22230 4438538..4439995 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
E3165_RS22235 4440052..4440609 - 558 WP_023438539.1 peptide chain release factor H -
E3165_RS22240 4440521..4440787 - 267 WP_001303804.1 hypothetical protein -
E3165_RS22245 4441094..4441546 - 453 WP_001059871.1 GNAT family N-acetyltransferase -
E3165_RS22250 4441556..4441954 - 399 WP_001263495.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
E3165_RS22255 4441957..4442250 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
E3165_RS22260 4442302..4443357 - 1056 WP_001226186.1 DNA polymerase IV -
E3165_RS22265 4443428..4444213 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
E3165_RS22270 4444158..4445897 + 1740 WP_001301901.1 flagellar biosynthesis protein FlhA -
E3165_RS22275 4446042..4446539 - 498 Protein_4367 REP-associated tyrosine transposase RayT -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4422407..4442250 19843


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-130)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 133 a.a.        Molecular weight: 15473.86 Da        Isoelectric Point: 8.0949

>T121604 WP_001263495.1 NZ_CP038336:c4441954-4441556 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T121604 NZ_CP038336:c4441954-4441556 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT121604 WP_000554757.1 NZ_CP038336:c4442250-4441957 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT121604 NZ_CP038336:c4442250-4441957 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References