Detailed information of TA system TA121598

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3494451..3494676
• Replicon: chromosome
• Accession: NZ_CP038336
• Organism: Escherichia coli O157:H7 strain LSU61

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E3165_RS17810
• Protein ID: WP_000813263.1
• Coordinates: 3494451..3494606 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3494618..3494676 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3165_RS17775	3489905..3490618	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
E3165_RS17780	3490756..3490952	-	197	Protein_3486	TrmB family transcriptional regulator	-
E3165_RS17785	3491239..3492057	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
E3165_RS17790	3492209..3492580	-	372	WP_000090264.1	antitermination protein	-
E3165_RS17795	3492570..3492941	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
E3165_RS17800	3492954..3494003	-	1050	WP_171877335.1	DUF968 domain-containing protein	-
E3165_RS17805	3494005..3494283	-	279	WP_001341388.1	hypothetical protein	-
E3165_RS17810	3494451..3494606	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3494618..3494676	+	59	-	-	Antitoxin
E3165_RS17815	3494708..3494845	+	138	WP_000955173.1	hypothetical protein	-
E3165_RS17820	3495211..3495984	+	774	WP_000160654.1	alpha/beta hydrolase	-
E3165_RS17825	3496336..3496749	-	414	WP_001151233.1	DUF977 family protein	-
E3165_RS17830	3496765..3497535	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
E3165_RS17835	3497557..3498303	-	747	WP_000788751.1	ATP-binding protein	-
E3165_RS17840	3498310..3499401	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T121598 WP_000813263.1 NZ_CP038336:c3494606-3494451 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T121598 NZ_CP038336:c3494606-3494451 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT121598 NZ_CP038336:3494618-3494676 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin