Detailed information of TA system TA121594

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2655506..2655731
• Replicon: chromosome
• Accession: NZ_CP038336
• Organism: Escherichia coli O157:H7 strain LSU61

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E3165_RS13290
• Protein ID: WP_000935259.1
• Coordinates: 2655519..2655731 (+)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2655506..2655564 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3165_RS13240	2650571..2650813	+	243	WP_171877255.1	hypothetical protein	-
E3165_RS13245	2650797..2651222	+	426	WP_000693816.1	Rha family transcriptional regulator	-
E3165_RS13250	2651291..2652334	+	1044	WP_057711673.1	phage replisome organizer	-
E3165_RS13255	2652327..2652788	+	462	WP_000139447.1	replication protein	-
E3165_RS13260	2652822..2653538	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
E3165_RS13265	2653571..2653852	+	282	WP_000603384.1	DNA-binding protein	-
E3165_RS13270	2653849..2654076	+	228	WP_000699809.1	hypothetical protein	-
E3165_RS13275	2654069..2654380	+	312	WP_001289673.1	hypothetical protein	-
E3165_RS13280	2654508..2654726	+	219	WP_000683609.1	DUF4014 family protein	-
E3165_RS13285	2654728..2655285	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2655506..2655564	-	59	-	-	Antitoxin
E3165_RS13290	2655519..2655731	+	213	WP_000935259.1	type I toxin-antitoxin system Hok family toxin	Toxin
E3165_RS13295	2655851..2656195	+	345	WP_171877256.1	YgiW/YdeI family stress tolerance OB fold protein	-
E3165_RS13300	2656317..2656589	+	273	WP_000191872.1	hypothetical protein	-
E3165_RS13305	2656591..2657640	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
E3165_RS13310	2657653..2658012	+	360	WP_001217447.1	RusA family crossover junction endodeoxyribonuclease	-
E3165_RS13315	2658021..2658575	+	555	WP_000640035.1	DUF1133 family protein	-
E3165_RS13320	2658800..2658997	+	198	WP_000917763.1	hypothetical protein	-
E3165_RS13325	2659134..2659847	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
E3165_RS13340	2660298..2660729	+	432	WP_001359877.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI / nleH2 / nleF / espM1 / paa / nleG7' / nleC	2530821..2698192	167371

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7887.47 Da
• Isoelectric Point: 9.2654

>T121594 WP_000935259.1 NZ_CP038336:2655519-2655731 [Escherichia coli O157:H7]
MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 213 bp

>T121594 NZ_CP038336:2655519-2655731 [Escherichia coli O157:H7]
ATGCTGAACACATGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTGCGAATCC
GAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT121594 NZ_CP038336:c2655564-2655506 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Antitoxin