Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 743983..744710 Replicon chromosome
Accession NZ_CP038336
Organism Escherichia coli O157:H7 strain LSU61

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag E3165_RS03650 Protein ID WP_000550193.1
Coordinates 743983..744297 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag E3165_RS03655 Protein ID WP_000560249.1
Coordinates 744294..744710 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3165_RS03630 740141..741127 - 987 WP_024201462.1 Gfo/Idh/MocA family oxidoreductase -
E3165_RS03635 741206..741898 - 693 WP_000942542.1 vancomycin high temperature exclusion protein -
E3165_RS03640 741975..742478 - 504 WP_001295542.1 M48 family metallopeptidase -
E3165_RS03645 742563..743699 + 1137 WP_000018678.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
E3165_RS03650 743983..744297 + 315 WP_000550193.1 type II toxin-antitoxin system toxin HigB Toxin
E3165_RS03655 744294..744710 + 417 WP_000560249.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
E3165_RS03660 744755..746773 - 2019 WP_000121479.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
E3165_RS03665 747299..749650 - 2352 WP_000695518.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12093.06 Da        Isoelectric Point: 10.0409

>T121582 WP_000550193.1 NZ_CP038336:743983-744297 [Escherichia coli O157:H7]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFSSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T121582 NZ_CP038336:743983-744297 [Escherichia coli O157:H7]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTAGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCTCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTTTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15016.48 Da        Isoelectric Point: 4.5805

>AT121582 WP_000560249.1 NZ_CP038336:744294-744710 [Escherichia coli O157:H7]
MIAIADILHAGEKLTAVAPFLAGIQNEEQYIQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT121582 NZ_CP038336:744294-744710 [Escherichia coli O157:H7]
ATGATTGCGATTGCCGACATCTTGCATGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACATCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References