Detailed information of TA system TA121581

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 701144..701943
• Replicon: chromosome
• Accession: NZ_CP038336
• Organism: Escherichia coli O157:H7 strain LSU61

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: E3165_RS03430
• Protein ID: WP_000347273.1
• Coordinates: 701144..701608 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: E3165_RS03435
• Protein ID: WP_001302819.1
• Coordinates: 701608..701943 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3165_RS03400	696145..696579	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
E3165_RS03405	696597..697475	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
E3165_RS03410	697465..698244	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
E3165_RS03415	698255..698728	-	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
E3165_RS03420	698751..700031	-	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
E3165_RS03425	700280..701089	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
E3165_RS03430	701144..701608	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
E3165_RS03435	701608..701943	-	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
E3165_RS03440	702092..703663	-	1572	WP_001273776.1	galactarate dehydratase	-
E3165_RS03445	704038..705372	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
E3165_RS03450	705388..706158	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T121581 WP_000347273.1 NZ_CP038336:c701608-701144 [Escherichia coli O157:H7]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T121581 NZ_CP038336:c701608-701144 [Escherichia coli O157:H7]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT121581 WP_001302819.1 NZ_CP038336:c701943-701608 [Escherichia coli O157:H7]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT121581 NZ_CP038336:c701943-701608 [Escherichia coli O157:H7]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7