Detailed information of TA system TA121567

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 4472173..4472712
• Replicon: chromosome
• Accession: NZ_CP038333
• Organism: Escherichia coli O157:H7 strain N8B7-2

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: E4U68_RS22685
• Protein ID: WP_001303998.1
• Coordinates: 4472452..4472712 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: C3TP07
• Locus tag: E4U68_RS22680
• Protein ID: WP_000729705.1
• Coordinates: 4472173..4472433 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U68_RS22660	4467927..4468712	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
E4U68_RS22665	4468657..4470396	+	1740	WP_001301901.1	flagellar biosynthesis protein FlhA	-
E4U68_RS22670	4470541..4471038	-	498	Protein_4437	REP-associated tyrosine transposase RayT	-
E4U68_RS22675	4471214..4471987	-	774	WP_000543891.1	C40 family peptidase	-
E4U68_RS22680	4472173..4472433	+	261	WP_000729705.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
E4U68_RS22685	4472452..4472712	+	261	WP_001303998.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
E4U68_RS22690	4472868..4473608	+	741	WP_001225679.1	murein L,D-transpeptidase	-
E4U68_RS22695	4473579..4474346	-	768	WP_001301698.1	class II glutamine amidotransferase	-
E4U68_RS22700	4474451..4475029	-	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-85)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10026.71 Da
• Isoelectric Point: 10.3188

>T121567 WP_001303998.1 NZ_CP038333:4472452-4472712 [Escherichia coli O157:H7]
MNSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGT
HAALFG

Nucleotide

• Length: 261 bp

>T121567 NZ_CP038333:4472452-4472712 [Escherichia coli O157:H7]
TTGAACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAATAAATTGAAATATCTTAT
GACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAGGTTCATGGAAAGGTTATC
GCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGATTTGAGAGAACTGGAACT
CACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9377.74 Da
• Isoelectric Point: 5.0758

>AT121567 WP_000729705.1 NZ_CP038333:4472173-4472433 [Escherichia coli O157:H7]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Nucleotide

• Length: 261 bp

>AT121567 NZ_CP038333:4472173-4472433 [Escherichia coli O157:H7]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA