Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 13609..14266 Replicon plasmid pNE1092-3
Accession NZ_CP038330
Organism Escherichia coli O157:H7 strain NE 1092-2

Toxin (Protein)


Gene name higB Uniprot ID U3PDC3
Locus tag E4U73_RS28065 Protein ID WP_000270043.1
Coordinates 13916..14266 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag E4U73_RS28060 Protein ID WP_000124640.1
Coordinates 13609..13911 (-) Length 101 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U73_RS28015 9234..9662 + 429 WP_000591074.1 hypothetical protein -
E4U73_RS28020 9719..10078 + 360 WP_000422768.1 hypothetical protein -
E4U73_RS28025 10078..10524 + 447 WP_000919345.1 Fe3+-siderophore ABC transporter permease -
E4U73_RS28030 10521..11039 + 519 WP_000210756.1 nitrite reductase -
E4U73_RS28035 11039..11269 + 231 WP_000972663.1 hypothetical protein -
E4U73_RS28040 11256..12113 + 858 WP_001167032.1 hypothetical protein -
E4U73_RS28045 12344..12871 + 528 WP_001236377.1 thermonuclease family protein -
E4U73_RS28050 12929..13201 + 273 WP_001043047.1 HU family DNA-binding protein -
E4U73_RS28055 13289..13582 + 294 WP_001239997.1 chromosome segregation protein ParM -
E4U73_RS28060 13609..13911 - 303 WP_000124640.1 XRE family transcriptional regulator Antitoxin
E4U73_RS28065 13916..14266 - 351 WP_000270043.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E4U73_RS28070 14429..14977 + 549 WP_001061574.1 transcriptional regulator -
E4U73_RS28075 15318..15512 + 195 WP_000343597.1 hypothetical protein -
E4U73_RS28080 15523..15894 + 372 WP_000516916.1 hypothetical protein -
E4U73_RS28085 15887..16357 + 471 WP_001281821.1 hypothetical protein -
E4U73_RS28090 16372..16707 - 336 WP_000683476.1 hypothetical protein -
E4U73_RS28095 16804..17292 + 489 WP_001273096.1 DUF1643 domain-containing protein -
E4U73_RS28100 17295..17792 + 498 WP_000062185.1 hypothetical protein -
E4U73_RS28105 18007..18711 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
E4U73_RS28110 18776..18955 - 180 Protein_30 helix-turn-helix transcriptional regulator -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR - 1..93970 93970


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-106)

Antitoxin

(33-79)


Sequences


Toxin        


Download         Length: 117 a.a.        Molecular weight: 13328.21 Da        Isoelectric Point: 5.2421

>T121538 WP_000270043.1 NZ_CP038330:c14266-13916 [Escherichia coli O157:H7]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Download         Length: 351 bp

>T121538 NZ_CP038330:c14266-13916 [Escherichia coli O157:H7]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11006.73 Da        Isoelectric Point: 7.2036

>AT121538 WP_000124640.1 NZ_CP038330:c13911-13609 [Escherichia coli O157:H7]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Download         Length: 303 bp

>AT121538 NZ_CP038330:c13911-13609 [Escherichia coli O157:H7]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7G8AFB9


Antitoxin

Source ID Structure

References