Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type I Classification (family/domain) symER/SymE(toxin)
Location 4878051..4878463 Replicon chromosome
Accession NZ_CP038328
Organism Escherichia coli O157:H7 strain NE 1092-2

Toxin (Protein)


Gene name symE Uniprot ID Q8FA88
Locus tag E4U73_RS24560 Protein ID WP_000132630.1
Coordinates 4878122..4878463 (+) Length 114 a.a.

Antitoxin (RNA)


Gene name symR
Locus tag -
Coordinates 4878051..4878127 (-)

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U73_RS24550 4874678..4876147 + 1470 WP_001302468.1 type I restriction-modification system subunit M -
E4U73_RS24555 4876147..4877901 + 1755 WP_000110082.1 restriction endonuclease subunit S -
- 4878051..4878127 - 77 NuclAT_15 - Antitoxin
- 4878051..4878127 - 77 NuclAT_15 - Antitoxin
- 4878051..4878127 - 77 NuclAT_15 - Antitoxin
- 4878051..4878127 - 77 NuclAT_15 - Antitoxin
- 4878051..4878127 - 77 NuclAT_16 - Antitoxin
- 4878051..4878127 - 77 NuclAT_16 - Antitoxin
- 4878051..4878127 - 77 NuclAT_16 - Antitoxin
- 4878051..4878127 - 77 NuclAT_16 - Antitoxin
E4U73_RS24560 4878122..4878463 + 342 WP_000132630.1 endoribonuclease SymE Toxin
E4U73_RS24565 4878510..4879673 - 1164 WP_001304006.1 DUF1524 domain-containing protein -
E4U73_RS24570 4879721..4880602 - 882 WP_001304007.1 DUF262 domain-containing protein -
E4U73_RS24575 4880699..4880863 - 165 WP_000394274.1 DUF1127 domain-containing protein -
E4U73_RS24580 4881040..4882452 + 1413 WP_000199295.1 PLP-dependent aminotransferase family protein -
E4U73_RS24585 4882695..4882895 - 201 WP_001310455.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - espX6 4870250..4891399 21149


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(20-71)


Sequences


Toxin        


Download         Length: 114 a.a.        Molecular weight: 12294.13 Da        Isoelectric Point: 8.4982

>T121533 WP_000132630.1 NZ_CP038328:4878122-4878463 [Escherichia coli O157:H7]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Download         Length: 342 bp

>T121533 NZ_CP038328:4878122-4878463 [Escherichia coli O157:H7]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin


Download         Length: 77 bp

>AT121533 NZ_CP038328:c4878127-4878051 [Escherichia coli O157:H7]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0E0Y5T0


Antitoxin

Download structure file

References