Detailed information of TA system TA121427

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3496376..3496601
• Replicon: chromosome
• Accession: NZ_CP038319
• Organism: Escherichia coli O157:H7 strain NE122

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E3167_RS17755
• Protein ID: WP_000813263.1
• Coordinates: 3496376..3496531 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3496543..3496601 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3167_RS17720	3491830..3492543	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
E3167_RS17725	3492681..3492877	-	197	Protein_3469	TrmB family transcriptional regulator	-
E3167_RS17730	3493164..3493982	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
E3167_RS17735	3494134..3494505	-	372	WP_000090264.1	antitermination protein	-
E3167_RS17740	3494495..3494866	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
E3167_RS17745	3494879..3495928	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
E3167_RS17750	3495930..3496208	-	279	WP_001341388.1	hypothetical protein	-
E3167_RS17755	3496376..3496531	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3496543..3496601	+	59	-	-	Antitoxin
E3167_RS17760	3496633..3496770	+	138	WP_000955173.1	hypothetical protein	-
E3167_RS17765	3497136..3497909	+	774	WP_000160654.1	alpha/beta hydrolase	-
E3167_RS17770	3498261..3498674	-	414	WP_001151233.1	DUF977 family protein	-
E3167_RS17775	3498690..3499460	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
E3167_RS17780	3499482..3500228	-	747	WP_000788745.1	ATP-binding protein	-
E3167_RS17785	3500235..3501320	-	1086	WP_072141648.1	DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T121427 WP_000813263.1 NZ_CP038319:c3496531-3496376 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T121427 NZ_CP038319:c3496531-3496376 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT121427 NZ_CP038319:3496543-3496601 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin