Detailed information of TA system TA121422

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2575039..2575264
• Replicon: chromosome
• Accession: NZ_CP038319
• Organism: Escherichia coli O157:H7 strain NE122

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E3167_RS12985
• Protein ID: WP_000935259.1
• Coordinates: 2575052..2575264 (+)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2575039..2575097 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3167_RS12935	2570092..2570334	+	243	WP_000747948.1	hypothetical protein	-
E3167_RS12940	2570318..2570743	+	426	WP_000693878.1	Rha family transcriptional regulator	-
E3167_RS12945	2570812..2571867	+	1056	WP_001356791.1	hypothetical protein	-
E3167_RS12950	2571860..2572321	+	462	WP_000139447.1	replication protein	-
E3167_RS12955	2572355..2573071	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
E3167_RS12960	2573104..2573385	+	282	WP_000603384.1	DNA-binding protein	-
E3167_RS12965	2573382..2573609	+	228	WP_000699809.1	hypothetical protein	-
E3167_RS12970	2573602..2573913	+	312	WP_001289673.1	hypothetical protein	-
E3167_RS12975	2574041..2574259	+	219	WP_000683609.1	DUF4014 family protein	-
E3167_RS12980	2574261..2574818	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2575039..2575097	-	59	-	-	Antitoxin
E3167_RS12985	2575052..2575264	+	213	WP_000935259.1	type I toxin-antitoxin system Hok family toxin	Toxin
E3167_RS12990	2575384..2575728	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
E3167_RS12995	2575850..2576122	+	273	WP_000191871.1	hypothetical protein	-
E3167_RS13000	2576124..2577173	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
E3167_RS13005	2577186..2577491	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
E3167_RS13010	2577554..2578108	+	555	WP_000640035.1	DUF1133 family protein	-
E3167_RS13015	2578333..2578530	+	198	WP_000917763.1	hypothetical protein	-
E3167_RS13020	2578666..2579379	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
E3167_RS13035	2579830..2580261	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2514551..2619610	105059

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7887.47 Da
• Isoelectric Point: 9.2654

>T121422 WP_000935259.1 NZ_CP038319:2575052-2575264 [Escherichia coli O157:H7]
MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 213 bp

>T121422 NZ_CP038319:2575052-2575264 [Escherichia coli O157:H7]
ATGCTGAACACATGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTGCGAATCC
GAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT121422 NZ_CP038319:c2575097-2575039 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Antitoxin