Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4368628..4369167 Replicon chromosome
Accession NZ_CP038316
Organism Escherichia coli O157:H7 strain NE92

Toxin (Protein)


Gene name yafQ Uniprot ID -
Locus tag E3166_RS21780 Protein ID WP_001303998.1
Coordinates 4368907..4369167 (+) Length 87 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID C3TP07
Locus tag E3166_RS21775 Protein ID WP_000729705.1
Coordinates 4368628..4368888 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3166_RS21755 4364382..4365167 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
E3166_RS21760 4365112..4366851 + 1740 WP_001301901.1 flagellar biosynthesis protein FlhA -
E3166_RS21765 4366996..4367493 - 498 Protein_4261 REP-associated tyrosine transposase RayT -
E3166_RS21770 4367669..4368442 - 774 WP_000543891.1 C40 family peptidase -
E3166_RS21775 4368628..4368888 + 261 WP_000729705.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
E3166_RS21780 4368907..4369167 + 261 WP_001303998.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
E3166_RS21785 4369323..4370063 + 741 WP_001225679.1 murein L,D-transpeptidase -
E3166_RS21790 4370034..4370801 - 768 WP_001301698.1 class II glutamine amidotransferase -
E3166_RS21795 4370906..4371484 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-85)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 87 a.a.        Molecular weight: 10026.71 Da        Isoelectric Point: 10.3188

>T121395 WP_001303998.1 NZ_CP038316:4368907-4369167 [Escherichia coli O157:H7]
MNSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGT
HAALFG

Download         Length: 261 bp

>T121395 NZ_CP038316:4368907-4369167 [Escherichia coli O157:H7]
TTGAACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAATAAATTGAAATATCTTAT
GACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAGGTTCATGGAAAGGTTATC
GCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGATTTGAGAGAACTGGAACT
CACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9377.74 Da        Isoelectric Point: 5.0758

>AT121395 WP_000729705.1 NZ_CP038316:4368628-4368888 [Escherichia coli O157:H7]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Download         Length: 261 bp

>AT121395 NZ_CP038316:4368628-4368888 [Escherichia coli O157:H7]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References