Detailed information of TA system TA121313

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3038416..3038641
• Replicon: chromosome
• Accession: NZ_CP038309
• Organism: Escherichia coli O157:H7 strain Show KS 470-1

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E4U84_RS15435
• Protein ID: WP_000967408.1
• Coordinates: 3038416..3038628 (-)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3038583..3038641 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U84_RS15400	3033719..3034777	-	1059	WP_000483497.1	site-specific DNA-methyltransferase	-
E4U84_RS15405	3034928..3035125	-	198	WP_000917735.1	hypothetical protein	-
E4U84_RS15410	3035352..3036173	-	822	WP_000762904.1	antitermination protein	-
E4U84_RS15415	3036170..3036544	-	375	WP_000904137.1	RusA family crossover junction endodeoxyribonuclease	-
E4U84_RS15420	3036557..3037606	-	1050	Protein_3017	DUF968 domain-containing protein	-
E4U84_RS15425	3037608..3037886	-	279	WP_001304183.1	hypothetical protein	-
E4U84_RS15430	3037952..3038119	-	168	WP_000998188.1	hypothetical protein	-
E4U84_RS15435	3038416..3038628	-	213	WP_000967408.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	3038583..3038641	+	59	-	-	Antitoxin
E4U84_RS15440	3038817..3038921	-	105	WP_001278450.1	hypothetical protein	-
E4U84_RS15445	3039037..3039399	-	363	WP_000610379.1	DUF551 domain-containing protein	-
E4U84_RS15450	3039396..3039767	-	372	WP_000137941.1	hypothetical protein	-
E4U84_RS15455	3039803..3040015	-	213	WP_000063625.1	hypothetical protein	-
E4U84_RS15460	3040064..3040420	-	357	WP_001302146.1	hypothetical protein	-
E4U84_RS15465	3040477..3040872	-	396	WP_001118161.1	DUF977 family protein	-
E4U84_RS15470	3040888..3041658	-	771	WP_000450888.1	DUF1627 domain-containing protein	-
E4U84_RS15475	3041688..3042428	-	741	WP_000790456.1	ATP-binding protein	-
E4U84_RS15480	3042435..3043388	-	954	WP_000095667.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	espM1 / nleA/espI / paa	3003473..3112354	108881

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7958.54 Da
• Isoelectric Point: 9.6297

>T121313 WP_000967408.1 NZ_CP038309:c3038628-3038416 [Escherichia coli O157:H7]
MLYTRRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 213 bp

>T121313 NZ_CP038309:c3038628-3038416 [Escherichia coli O157:H7]
ATGCTCTACACACGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGCT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTACGAATCC
GAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT121313 NZ_CP038309:3038583-3038641 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Antitoxin