Detailed information of TA system TA121273

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3540739..3540964
• Replicon: chromosome
• Accession: NZ_CP038305
• Organism: Escherichia coli O157:H7 strain SS NE 1040-1

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E4U78_RS18245
• Protein ID: WP_000813263.1
• Coordinates: 3540739..3540894 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3540906..3540964 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U78_RS18210	3536193..3536906	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
E4U78_RS18215	3537044..3537240	-	197	Protein_3565	TrmB family transcriptional regulator	-
E4U78_RS18220	3537527..3538345	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
E4U78_RS18225	3538497..3538868	-	372	WP_000090264.1	antitermination protein	-
E4U78_RS18230	3538858..3539229	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
E4U78_RS18235	3539242..3540291	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
E4U78_RS18240	3540293..3540571	-	279	WP_001341388.1	hypothetical protein	-
E4U78_RS18245	3540739..3540894	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3540906..3540964	+	59	-	-	Antitoxin
E4U78_RS18250	3540996..3541133	+	138	WP_000955173.1	hypothetical protein	-
E4U78_RS18255	3541499..3542272	+	774	WP_000160654.1	alpha/beta hydrolase	-
E4U78_RS18260	3542624..3543037	-	414	WP_001151233.1	DUF977 family protein	-
E4U78_RS18265	3543053..3543823	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
E4U78_RS18270	3543845..3544591	-	747	WP_000788751.1	ATP-binding protein	-
E4U78_RS18275	3544598..3545689	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T121273 WP_000813263.1 NZ_CP038305:c3540894-3540739 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T121273 NZ_CP038305:c3540894-3540739 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT121273 NZ_CP038305:3540906-3540964 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin