Detailed information of TA system TA121270

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2973022..2973247
• Replicon: chromosome
• Accession: NZ_CP038305
• Organism: Escherichia coli O157:H7 strain SS NE 1040-1

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E4U78_RS15020
• Protein ID: WP_000935259.1
• Coordinates: 2973022..2973234 (-)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2973189..2973247 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U78_RS14970	2968025..2968456	-	432	WP_001302123.1	tellurite resistance protein	-
E4U78_RS14985	2968907..2969620	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
E4U78_RS14990	2969756..2969953	-	198	WP_000917763.1	hypothetical protein	-
E4U78_RS14995	2970178..2970732	-	555	WP_000640035.1	DUF1133 family protein	-
E4U78_RS15000	2970795..2971100	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
E4U78_RS15005	2971113..2972162	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
E4U78_RS15010	2972164..2972436	-	273	WP_000191872.1	hypothetical protein	-
E4U78_RS15015	2972558..2972902	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
E4U78_RS15020	2973022..2973234	-	213	WP_000935259.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2973189..2973247	+	59	-	-	Antitoxin
E4U78_RS15025	2973468..2974025	-	558	WP_000104474.1	DUF551 domain-containing protein	-
E4U78_RS15030	2974027..2974245	-	219	WP_000683609.1	DUF4014 family protein	-
E4U78_RS15035	2974373..2974684	-	312	WP_001289673.1	hypothetical protein	-
E4U78_RS15040	2974677..2974904	-	228	WP_000699809.1	hypothetical protein	-
E4U78_RS15045	2974901..2975182	-	282	WP_000603384.1	DNA-binding protein	-
E4U78_RS15050	2975215..2975931	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
E4U78_RS15055	2975965..2976426	-	462	WP_000139447.1	replication protein	-
E4U78_RS15060	2976419..2977462	-	1044	WP_001262402.1	hypothetical protein	-
E4U78_RS15065	2977531..2977956	-	426	WP_000693878.1	Rha family transcriptional regulator	-
E4U78_RS15070	2977940..2978182	-	243	WP_000747948.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	espM1 / nleA/espI / paa	2935911..3048030	112119

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7887.47 Da
• Isoelectric Point: 9.2654

>T121270 WP_000935259.1 NZ_CP038305:c2973234-2973022 [Escherichia coli O157:H7]
MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 213 bp

>T121270 NZ_CP038305:c2973234-2973022 [Escherichia coli O157:H7]
ATGCTGAACACATGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTGCGAATCC
GAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT121270 NZ_CP038305:2973189-2973247 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Antitoxin