Detailed information of TA system TA121231

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2771804..2772175
• Replicon: chromosome
• Accession: NZ_CP038302
• Organism: Escherichia coli O157:H7 strain SS TX 313-1

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A891STD1
• Locus tag: E4U66_RS14085
• Protein ID: WP_042853000.1
• Coordinates: 2771981..2772175 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2771804..2771982 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U66_RS14055	2767556..2767726	+	171	WP_001625136.1	hypothetical protein	-
E4U66_RS14060	2767759..2769132	+	1374	WP_000123746.1	ATP-dependent RNA helicase DbpA	-
E4U66_RS14065	2769261..2770196	-	936	WP_001157382.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
E4U66_RS14070	2770248..2771483	-	1236	WP_000040845.1	site-specific integrase	-
E4U66_RS14075	2771485..2771700	-	216	WP_000079604.1	excisionase XisR	-
E4U66_RS14080	2771800..2771988	-	189	WP_001356607.1	DUF1187 family protein	-
-	2771804..2771982	+	179	NuclAT_0	-	Antitoxin
-	2771804..2771982	+	179	NuclAT_0	-	Antitoxin
-	2771804..2771982	+	179	NuclAT_0	-	Antitoxin
-	2771804..2771982	+	179	NuclAT_0	-	Antitoxin
E4U66_RS14085	2771981..2772175	-	195	WP_042853000.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
E4U66_RS14090	2772232..2773041	-	810	WP_000166315.1	recombination protein RecT	-
E4U66_RS14095	2773034..2775703	-	2670	WP_000102194.1	exodeoxyribonuclease VIII	-
E4U66_RS14100	2775784..2775960	-	177	WP_000245534.1	hypothetical protein	-
E4U66_RS14105	2775954..2776175	-	222	WP_000560226.1	killing protein KilR	-
E4U66_RS14110	2776222..2777070	-	849	WP_000887681.1	Rha family phage regulatory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2770248..2823461	53213

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7019.87 Da
• Isoelectric Point: 8.6419

>T121231 WP_042853000.1 NZ_CP038302:c2772175-2771981 [Escherichia coli O157:H7]
MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T121231 NZ_CP038302:c2772175-2771981 [Escherichia coli O157:H7]
ATGAGATATGACGATGTTAAACCATGTCCGTTTTGTGGTTGTCCATCTGTTACGGTGAAAGCAATTTCAGGATATTACCG
GGCAAAATGCAACGGATGCGAATCCCGAACTGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CTACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT121231 NZ_CP038302:2771804-2771982 [Escherichia coli O157:H7]
GAGGACAGAAGTTTCTCGCAATTAAAATTTATCAGCTTTACTTTCTGCTCTCTGGACACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAATTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCTTTTT
CAATAGTGGCAGTTATTTT

Structures

Antitoxin