Detailed information of TA system TA121196

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2746069..2746440
• Replicon: chromosome
• Accession: NZ_CP038300
• Organism: Escherichia coli O157:H7 strain SS TX 754-1

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A891STD1
• Locus tag: E4U76_RS13885
• Protein ID: WP_042853000.1
• Coordinates: 2746246..2746440 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2746069..2746247 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U76_RS13855	2741821..2741991	+	171	WP_001625136.1	hypothetical protein	-
E4U76_RS13860	2742024..2743397	+	1374	WP_000123746.1	ATP-dependent RNA helicase DbpA	-
E4U76_RS13865	2743526..2744461	-	936	WP_001157382.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
E4U76_RS13870	2744513..2745748	-	1236	WP_000040845.1	site-specific integrase	-
E4U76_RS13875	2745750..2745965	-	216	WP_000079604.1	excisionase XisR	-
E4U76_RS13880	2746065..2746253	-	189	WP_001356607.1	DUF1187 family protein	-
-	2746069..2746247	+	179	NuclAT_0	-	Antitoxin
-	2746069..2746247	+	179	NuclAT_0	-	Antitoxin
-	2746069..2746247	+	179	NuclAT_0	-	Antitoxin
-	2746069..2746247	+	179	NuclAT_0	-	Antitoxin
E4U76_RS13885	2746246..2746440	-	195	WP_042853000.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
E4U76_RS13890	2746497..2747306	-	810	WP_000166315.1	recombination protein RecT	-
E4U76_RS13895	2747299..2749968	-	2670	WP_000102194.1	exodeoxyribonuclease VIII	-
E4U76_RS13900	2750049..2750225	-	177	WP_000245534.1	hypothetical protein	-
E4U76_RS13905	2750219..2750440	-	222	WP_000560226.1	killing protein KilR	-
E4U76_RS13910	2750487..2751335	-	849	WP_000887681.1	Rha family phage regulatory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2744513..2787296	42783

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7019.87 Da
• Isoelectric Point: 8.6419

>T121196 WP_042853000.1 NZ_CP038300:c2746440-2746246 [Escherichia coli O157:H7]
MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T121196 NZ_CP038300:c2746440-2746246 [Escherichia coli O157:H7]
ATGAGATATGACGATGTTAAACCATGTCCGTTTTGTGGTTGTCCATCTGTTACGGTGAAAGCAATTTCAGGATATTACCG
GGCAAAATGCAACGGATGCGAATCCCGAACTGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CTACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT121196 NZ_CP038300:2746069-2746247 [Escherichia coli O157:H7]
GAGGACAGAAGTTTCTCGCAATTAAAATTTATCAGCTTTACTTTCTGCTCTCTGGACACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAATTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCTTTTT
CAATAGTGGCAGTTATTTT

Structures

Antitoxin