Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4178766..4179460 Replicon chromosome
Accession NZ_CP038295
Organism Escherichia coli O157:H7 strain TB182A

Toxin (Protein)


Gene name yafO Uniprot ID C3TNX7
Locus tag E3171_RS20375 Protein ID WP_001263495.1
Coordinates 4178766..4179164 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag E3171_RS20380 Protein ID WP_000554757.1
Coordinates 4179167..4179460 (-) Length 98 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 4174353..4174433 - 81 NuclAT_11 - -
- 4174353..4174433 - 81 NuclAT_11 - -
- 4174353..4174433 - 81 NuclAT_11 - -
- 4174353..4174433 - 81 NuclAT_11 - -
E3171_RS20350 4175029..4175487 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
E3171_RS20355 4175748..4177205 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
E3171_RS20360 4177262..4177819 - 558 WP_023438539.1 peptide chain release factor H -
E3171_RS20365 4177731..4177997 - 267 WP_001303804.1 hypothetical protein -
E3171_RS20370 4178304..4178756 - 453 WP_001059871.1 GNAT family N-acetyltransferase -
E3171_RS20375 4178766..4179164 - 399 WP_001263495.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
E3171_RS20380 4179167..4179460 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
E3171_RS20385 4179512..4180567 - 1056 WP_001226186.1 DNA polymerase IV -
E3171_RS20390 4180638..4181423 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
E3171_RS20395 4181368..4181712 + 345 Protein_3995 FHIPEP family type III secretion protein -
E3171_RS20400 4182009..4182506 - 498 Protein_3996 REP-associated tyrosine transposase RayT -
E3171_RS20405 4182682..4183455 - 774 WP_000543891.1 C40 family peptidase -
E3171_RS20410 4183641..4183901 + 261 WP_000729705.1 type II toxin-antitoxin system antitoxin DinJ -
E3171_RS20415 4183904..4184182 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - espK / espK / nleB2 / espM2 / espW / flhA 4105057..4183455 78398


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-130)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 133 a.a.        Molecular weight: 15473.86 Da        Isoelectric Point: 8.0949

>T121171 WP_001263495.1 NZ_CP038295:c4179164-4178766 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T121171 NZ_CP038295:c4179164-4178766 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT121171 WP_000554757.1 NZ_CP038295:c4179460-4179167 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT121171 NZ_CP038295:c4179460-4179167 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References