Detailed information of TA system TA121154

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 700260..701059
• Replicon: chromosome
• Accession: NZ_CP038295
• Organism: Escherichia coli O157:H7 strain TB182A

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: E3171_RS03425
• Protein ID: WP_000347273.1
• Coordinates: 700260..700724 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: E3171_RS03430
• Protein ID: WP_001302819.1
• Coordinates: 700724..701059 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3171_RS03395	695261..695695	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
E3171_RS03400	695713..696591	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
E3171_RS03405	696581..697360	-	780	WP_000406210.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
E3171_RS03410	697371..697844	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
E3171_RS03415	697867..699147	-	1281	WP_000681946.1	tagatose-bisphosphate aldolase subunit KbaZ	-
E3171_RS03420	699396..700205	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
E3171_RS03425	700260..700724	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
E3171_RS03430	700724..701059	-	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
E3171_RS03435	701208..702779	-	1572	WP_001273775.1	galactarate dehydratase	-
E3171_RS03440	703154..704488	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
E3171_RS03445	704504..705274	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T121154 WP_000347273.1 NZ_CP038295:c700724-700260 [Escherichia coli O157:H7]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T121154 NZ_CP038295:c700724-700260 [Escherichia coli O157:H7]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT121154 WP_001302819.1 NZ_CP038295:c701059-700724 [Escherichia coli O157:H7]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT121154 NZ_CP038295:c701059-700724 [Escherichia coli O157:H7]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7