121145

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) symER/SymE(toxin)
Location 4807260..4807672 Replicon chromosome
Accession NZ_CP038292
Organism Escherichia coli O157:H7 strain TB21-1

Toxin (Protein)


Gene name symE Uniprot ID Q8FA88
Locus tag E4U65_RS24125 Protein ID WP_000132630.1
Coordinates 4807331..4807672 (+) Length 114 a.a.

Antitoxin (RNA)


Gene name symR
Locus tag -
Coordinates 4807260..4807336 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U65_RS24115 4803887..4805356 + 1470 WP_001302468.1 type I restriction-modification system subunit M -
E4U65_RS24120 4805356..4807110 + 1755 WP_000110082.1 restriction endonuclease subunit S -
- 4807260..4807336 - 77 NuclAT_14 - Antitoxin
- 4807260..4807336 - 77 NuclAT_14 - Antitoxin
- 4807260..4807336 - 77 NuclAT_14 - Antitoxin
- 4807260..4807336 - 77 NuclAT_14 - Antitoxin
- 4807260..4807336 - 77 NuclAT_15 - Antitoxin
- 4807260..4807336 - 77 NuclAT_15 - Antitoxin
- 4807260..4807336 - 77 NuclAT_15 - Antitoxin
- 4807260..4807336 - 77 NuclAT_15 - Antitoxin
E4U65_RS24125 4807331..4807672 + 342 WP_000132630.1 endoribonuclease SymE Toxin
E4U65_RS24130 4807719..4808882 - 1164 WP_001304006.1 DUF1524 domain-containing protein -
E4U65_RS24135 4808930..4809811 - 882 WP_001304007.1 DUF262 domain-containing protein -
E4U65_RS24140 4809908..4810072 - 165 WP_000394274.1 DUF1127 domain-containing protein -
E4U65_RS24145 4810249..4811517 + 1269 WP_171878124.1 aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme -
E4U65_RS24150 4811760..4811960 - 201 WP_001310455.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - espX6 4799459..4820533 21074


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SymE-like

(20-71)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12294.13 Da        Isoelectric Point: 8.4982

>T121145 WP_000132630.1 NZ_CP038292:4807331-4807672 [Escherichia coli O157:H7]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Download         Length: 342 bp

>T121145 NZ_CP038292:4807331-4807672 [Escherichia coli O157:H7]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin


Download         Length: 77 bp

>AT121145 NZ_CP038292:c4807336-4807260 [Escherichia coli O157:H7]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y5T0

Antitoxin

Download structure file

References